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Direct analysis of the secretions of the potato cyst nematode Globodera rostochiensis

Published online by Cambridge University Press:  01 August 1999

L. ROBERTSON ,
W. M. ROBERTSON  and
J. T. JONES
L. ROBERTSON
Affiliation:
Department of Nematology, Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA Present address: Universidad Autonoma de Madrid, Departamento de Biologia, Laboratorio de fisiologia vegetal, Cantoblanco, 28049 Madrid, Spain.
W. M. ROBERTSON
Affiliation:
Department of Nematology, Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA
J. T. JONES
Affiliation:
Department of Nematology, Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA
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Abstract

Secretions were induced from second (invasive) stage juveniles (J2s) of the potato cyst nematode Globodera rostochiensis by exposing them to 5-methoxy-N,N-dimethyl tryptamine oxalate (DMT). Secretions were collected from J2s in sufficient quantity to allow direct analysis. Gel electrophoresis followed by monochromatic silver staining demonstrated the presence of at least 10 proteins. The presence of several enzymes, including superoxide dismutase and proteases, was demonstrated using Western blots and activity assays. Antisera raised against the secretions recognized bands on Western blots consistent in molecular mass with those identified on silver stained gels. The antisera recognized structures implicated in the production of secretions including the subventral gland cells and surface of J2s.

Keywords

Globodera rostochiensissecretionscellulasesuperoxide dismutaseproteaseantisera
Type
Research Article
Information
Parasitology , Volume 119 , Issue 2 , August 1999 , pp. 167 - 176
Copyright
1999 Cambridge University Press

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