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Direct molecular identification of Trypanosoma cruzi Discrete Typing Units in domestic and peridomestic Triatoma infestans and Triatoma sordida from the Argentine Chaco

Published online by Cambridge University Press:  19 July 2012

L. MAFFEY ,
M. V. CARDINAL ,
P. C. ORDÓÑEZ-KRASNOWSKI ,
L. A. LANATI ,
M. A. LAURICELLA ,
A. G. SCHIJMAN  and
R. E. GÜRTLER
L. MAFFEY
Affiliation:
Laboratorio de Eco-Epidemiología, Departamento de Ecología, Genética y Evolución, Universidad de Buenos Aires, Buenos Aires, Argentina
M. V. CARDINAL*
Affiliation:
Laboratorio de Eco-Epidemiología, Departamento de Ecología, Genética y Evolución, Universidad de Buenos Aires, Buenos Aires, Argentina
P. C. ORDÓÑEZ-KRASNOWSKI
Affiliation:
Laboratorio de Eco-Epidemiología, Departamento de Ecología, Genética y Evolución, Universidad de Buenos Aires, Buenos Aires, Argentina
L. A. LANATI
Affiliation:
Laboratorio de Eco-Epidemiología, Departamento de Ecología, Genética y Evolución, Universidad de Buenos Aires, Buenos Aires, Argentina
M. A. LAURICELLA
Affiliation:
Instituto Nacional de Parasitología‘‘Dr. Mario Fatala Chabén’’-ANLIS, Buenos Aires, Argentina
A. G. SCHIJMAN
Affiliation:
Laboratorio de Biología Molecular de la Enfermedad de Chagas, Instituto de Investigaciones en Ingeniería Genética y Biología Molecular (INGEBI-CONICET), Vuelta de Obligado 2490, Buenos Aires, Argentina
R. E. GÜRTLER
Affiliation:
Laboratorio de Eco-Epidemiología, Departamento de Ecología, Genética y Evolución, Universidad de Buenos Aires, Buenos Aires, Argentina
*
*Corresponding author: Laboratorio de Eco-Epidemiología, Departamento de Ecología, Genética y Evolución, Universidad de Buenos Aires, Ciudad Universitaria, 1428 Buenos Aires, Argentina. Tel/Fax: +54 11 4576 3318. E-mail:mvcardinal@ege.fcen.uba.ar
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Summary

We assessed the distribution of Trypanosoma cruzi Discrete Typing Units (DTUs) in domestic and peridomestic Triatoma infestans and Triatoma sordida specimens collected in a well-defined rural area in Pampa del Indio, northeastern Argentina. Microscopically-positive bugs were randomly selected with a multi-level sampling design, and DTUs were identified using direct PCR strategies. TcVI predominated in 61% of 69 T. infestans and in 56% of 9 T. sordida. TcV was the secondary DTU in T. infestans (16%) and was found in 1 T. sordida specimen (11%). Three T. sordida (33%) were found infected with TcI, a DTU also identified in local Didelphis albiventris opossums. Mixed DTU infections occurred rarely (5%) and were detected both directly from the bugs' rectal ampoule and parasite cultures. The identified DTUs and bug collection sites of T. infestans were significantly associated. Bugs infected with TcV were almost exclusively captured in domiciles whereas those with TcVI were found similarly in domiciles and peridomiciles. All mixed infections occurred in domiciles. TcV-infected bugs fed more often on humans than on dogs, whereas TcVI-infected bugs showed the reverse pattern. T. sordida is a probable sylvatic vector of TcI linked to D. albiventris, and could represent a secondary vector of TcVI and TcV in the domestic/peridomestic cycle.

Keywords

Trypanosoma cruziDiscrete Typing UnitsPCRTriatoma infestansTriatoma sordidaChagas disease
Type
Research Article
Information
Parasitology , Volume 139 , Issue 12 , October 2012 , pp. 1570 - 1579
Copyright
Copyright © Cambridge University Press 2012

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