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The distribution of motor proteins in the muscles and flame cells of the Schistosoma mansoni miracidium and primary sporocyst

Published online by Cambridge University Press:  02 June 2006

D. BAHIA ,
L. G. A. AVELAR ,
F. VIGOROSI ,
D. CIOLI ,
G. C. OLIVEIRA  and
R. A. MORTARA
D. BAHIA
Affiliation:
Laboratório de Parasitologia Celular e Molecular, Centro de Pesquisas René Rachou-FIOCRUZ, Av. Augusto de Lima 1715, 30190-002, Belo Horizonte, MG, Brazil Present address: Departamento de Genética, ESALQ, USP, Av. Pádua Dias, 11, 13400-970, Piracicaba, SP, Brazil.
L. G. A. AVELAR
Affiliation:
Laboratório de Parasitologia Celular e Molecular, Centro de Pesquisas René Rachou-FIOCRUZ, Av. Augusto de Lima 1715, 30190-002, Belo Horizonte, MG, Brazil
F. VIGOROSI
Affiliation:
Institute of Cell Biology, 32 Via Ramarini, 00016 Monterotondo, Rome, Italy
D. CIOLI
Affiliation:
Institute of Cell Biology, 32 Via Ramarini, 00016 Monterotondo, Rome, Italy
G. C. OLIVEIRA
Affiliation:
Laboratório de Parasitologia Celular e Molecular, Centro de Pesquisas René Rachou-FIOCRUZ, Av. Augusto de Lima 1715, 30190-002, Belo Horizonte, MG, Brazil
R. A. MORTARA
Affiliation:
Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, Universidade Federal de São Paulo, Rua Botucatu 862, 6 ° andar, 04023-062, São Paulo, SP, Brazil
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Abstract

Schistosoma mansoni eggs, miracidia and primary sporocysts were labelled with phalloidin-rhodamine to visualize filamentous actin structures. Analysis of these forms by confocal fluorescence microscopy revealed the presence of previously well-defined circular and longitudinal muscle layers. Besides these muscular layers that sustain and provide motility to these parasite forms, we found in these 3 consecutive developmental stages of the parasite previously unidentified actin-rich tubular structures. In the 3 forms, 4 actin-rich tubules could be observed by optical sectioning underneath the well-developed muscle layers. The tubules appear in pairs, transversal to the length of the parasite, and located towards the extremities. By using an anti-flame cell specific antibody we confirmed that the tubules co-localize with flame cells and also determined that the tubule core is filled with microtubules. The additional presence of myosin in these tubules strongly suggests that they are contractile structures.

Keywords

Schistosoma mansonieggmiracidiumsporocystflame cellsactinmyosintubulintubule
Type
Research Article
Information
Parasitology , Volume 133 , Issue 3 , September 2006 , pp. 321 - 329
Copyright
2006 Cambridge University Press

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