Hostname: page-component-78c5997874-4rdpn Total loading time: 0 Render date: 2024-11-17T20:40:37.919Z Has data issue: false hasContentIssue false
  • English
  • Français

Egg-shell permeability and hatching of Ascaris suum

Published online by Cambridge University Press:  06 April 2009

A. J. Clarke  and
R. N. Perry
A. J. Clarke
Affiliation:
Nematology Department, Rothamsted Experimental Station, Harpenden, Herts, AL5 2JQ
R. N. Perry
Affiliation:
Nematology Department, Rothamsted Experimental Station, Harpenden, Herts, AL5 2JQ
Get access Rights & Permissions [Opens in a new window]

Summary

When eggs of Ascaris suum were transferred from glass-distilled water into 0·1–0·4 m NaCl solutions, the water content of the unhatched juveniles fell with increasing concentration of solute. The effect was reversible. The egg-shell was thus permeable to water. The osmotic pressure of the egg fluid was osmotically equivalent to between 0·1 and 0·2 m trehalose. In hatching experiments in Fairbairn's medium containing 0·1 or 0·2 m trehalose, only 5 and 3% respectively of the eggs hatched; 83% of the eggs hatched in the absence of trehalose. The evidence suggests that loss of solutes from the egg fluid permits an increase in the water content of the unhatched juveniles and that this may be responsible for ending their quiescence.

Type
Research Article
Information
Parasitology , Volume 80 , Issue 3 , June 1980 , pp. 447 - 456
Copyright
Copyright © Cambridge University Press 1980

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Arthur, E. J. & Sanborn, R. C. (1969). Osmotic and ionic regulation in nematodes. In Chemical Zoology, vol. 3 (ed. Florkin, M., Scheer, B. T. and Kidder, G. W.), pp. 431–48. New York and London: Academic Press.Google Scholar
Barrett, J. (1976). Studies on the induction of permeability in Ascaris lumbricoides eggs. Parasitology 73, 109–21.CrossRefGoogle ScholarPubMed
Bataillon, E. (1901). La pression osmotique et les grand problèmes de la biologie. Wilhelm Roux Archiv für Entwicklungsmechanik der Organismen 11, 149–85.CrossRefGoogle Scholar
Clarke, A. J. & Perry, R. N. (1977). Hatching of cyst nematodes. Nematologica 23, 350–68.Google Scholar
Clarke, A. J., Perry, R. N. & Hennessy, J. (1978). Osmotic stress and the hatching of Globodera rostochiensis. Nemotologica 24, 384–92.Google Scholar
Ellenby, C. (1968 a). Desiccation survival in the plant parasitic nematodes, Heterodera rostochiensis Wollenweber and Ditylenehus dipsaci (Kuhn) Filipjev. Proceedings of the Royal Society, B 169, 203–13.Google Scholar
Ellenby, C. (1968 b). Determination of the water content of nematode worms by interference microscopy. Experientia 24, 84–5.CrossRefGoogle ScholarPubMed
Ellenby, C. & Perry, R. N. (1976). The influence of the hatching factor on the water uptake of the second stage larva of the potato cyst nematode Heterodera rostochiensis. Journal of Experimental Biology 64, 141–7.CrossRefGoogle Scholar
Fairbairn, D. (1957). The biochemistry of Ascaris. Experimental Parasitology 6, 491–554.CrossRefGoogle ScholarPubMed
Fairbairn, D. (1961). The in vitro hatching of Ascaris lumbricoides eggs. Canadian Journal of Zoology 39, 153–62.CrossRefGoogle Scholar
Fairbairn, D. (1970). Physiological hatching of Ascaris lumbricoides eggs. In Experiments and Techniques in Parasitology (ed. MacInnis, A. J. and Voge, M.), pp. 21–3. San Francisco: W. H. Freeman.Google Scholar
Fairbairn, D. & Passey, B. I. (1955). The lipid components in the vitelline membrane of Ascaris lumbricoides eggs. Canadian Journal of Biochemistry and Physiology 33, 130–4.CrossRefGoogle ScholarPubMed
Fairbairn, D. & Passey, R. F. (1957). Occurrence and distribution of trehalose and glycogen in the eggs and tissues of Ascaris lumbricoides. Experimental Parasitology 6, 566–74.CrossRefGoogle Scholar
Hinck, L. W. & Ivey, M. H. (1976). Proteinase activity in Ascaris suum eggs, hatching fluid, and excretions-secretions. Journal of Parasitology 62, 771–4.CrossRefGoogle ScholarPubMed
Hobson, A. D., Stephenson, W. & Beadle, L. C. (1952). Studies on the physiology of Ascaris lumbricoides. I. The relation of the total osmotic pressure, conductivity and chloride content of the body fluid to that of the external environment. Journal of Experimental Biology 29, 1–21.CrossRefGoogle Scholar
Hobson, A. D., Stephenson, W. & Eden, A. (1952). Studies on the physiology of Ascaris lumbricoides. II. The inorganic composition of the body fluid in relation to that of the environment. Journal of Experimental Biology 29, 22–9.CrossRefGoogle Scholar
Jaskoski, B. J. & Colucci, A. V. (1964). In vitro hatching of Ascaris suum eggs. Transactions of the American Microscopical Society 83, 294–300.CrossRefGoogle Scholar
Justus, D. E. & Ivey, M. H. (1969). Chitinase activity in developmental stages of Ascaris suum and its inhibition by antibody. Journal of Parasitology 55, 472–6.CrossRefGoogle ScholarPubMed
Kaulenas, M. & Fairbairn, D. (1966). Induction of permeability in infective and uninfective eggs of Ascaris lumbricoides. Journal of Parasitology 52, 303–6.CrossRefGoogle Scholar
Kümmel, G., Dankwarth, L., Braun-Schubert, G. & Gertz, K.-H. (1969). Zur Struktur und Funktion des Exkretionssystems von Ascaris lumbricoides L. Zeitschrift für Vergleichende Physiologic 64, 118–34.CrossRefGoogle Scholar
Lee, D. L. & Atkinson, H. J. (1976). Physiology of Nematodes, 2nd edn.London and Basingstoke: Macmillan.CrossRefGoogle Scholar
Perry, R. N. (1978). Events in the hatching process of the potato-cyst nematode Globodera rostochiensis. A.R.C. Research Review 4, (3), 79–83.Google Scholar
Rogers, W. P. (1958). Physiology of the hatching of eggs of Ascaris lumbricoides. Nature, London 181, 1410–11.CrossRefGoogle Scholar
Rogers, W. P. (1960). The physiology of infective processes of nematode parasites; the stimulus from the animal host. Proceedings of the Royal Society, B 152, 367–86.Google ScholarPubMed
Rogers, W. P. & Sommerville, R. I. (1963). The infective stage of nematode parasites and its significance in parasitism. Advances in Parasitology 1, 109–77.CrossRefGoogle ScholarPubMed
Ward, K. A. & Fairbairn, D. (1972). Chitinase in developing eggs of Ascaris suum (Nematoda). Journal of Parasitology 58, 546–9.CrossRefGoogle ScholarPubMed
Wright, D. J. & Newall, D. R. (1976). Nitrogen excretion, osmotic and ionic regulation in nematodes. In The Organisation of Nematodes (ed. Croll, N.), pp. 163–210. New York and London: Academic Press.Google Scholar
Zawadowski, M. M. (1928). The nature of the egg-shell of various Ascaridae. [in Russian]. Transactions of the Laboratory of Experimental Biology, Zoopark, Moscow 4, 201–6.Google Scholar