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Eimeria tenella: experimental studies on the development of resistance to robenidine

Published online by Cambridge University Press:  06 April 2009

H. D. Chapman
H. D. Chapman
Affiliation:
Houghton Poultry Research Station, Houghton, Huntingdon, Cambs. PE17 2DA
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Extract

The development of resistance by the Houghton strain (H) of E. tenella to robenidine has been studied, in the laboratory, by serially passaging the strain in chickens fed increasing concentrations of drug. Resistance to robenidine developed more readily in experiments using larger numbers of birds with higher numbers of oocysts in the inoculum. Both these factors increased the parasite population and increased the chance of selecting parasites resistant to the drug. E. tenella (H) was made resistant to 264 ppm robenidine and showed no cross-resistance to other anticoccidial agents. Resistance arose in a series of ‘steps’ as the concentration of drug was increased. E. tenella (H) was continuously passaged at concentrations ranging from 2 to 33 ppm of robenidine. After 16 passages, lines passaged at 2, 4 and 8 ppm were not resistant to 33 ppm robenidine, suggesting that the degree of resistance developed was dependent upon the drug selection pressure.

Type
Research Article
Information
Parasitology , Volume 73 , Issue 3 , December 1976 , pp. 265 - 273
Copyright
Copyright © Cambridge University Press 1976

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References

Bryson, V. & Szybalski, W. (1955). Microbial drug resistance. Advances in Genetics 7, 1–46.CrossRefGoogle ScholarPubMed
Joyner, L. P. & Norton, C. C. (1974). Robenidine-dependence in a strain of Eimeria maxima. Parasitology 70, 47–51.CrossRefGoogle Scholar
Kantor, S., Kennett, R. L., Waletzky, E. & Tomcufcik, A. S. (1970). l,3-Bis-(p-chlorobenzylideneamino) guanidine hydrochloride (Robenzidene): New poultry anticoccidial agent. Science 168, 373–4.CrossRefGoogle Scholar
Long, P. L. (1972). Eimeria tenella: reproduction, pathogenicity and immunogenicity of a strain maintained in chick embryos by serial passage. Journal of Comparative Pathology 82, 429–37.CrossRefGoogle ScholarPubMed
McLoughlin, D. K. (1970). Coccidiosis: experimental analysis of drug resistance. Experimental Parasitology 28, 129–36.CrossRefGoogle ScholarPubMed
Norton, C. C. & Joyner, L. P. (1975). The development of drug resistant strains of Eimeria maxima in the laboratory. Parasitology 71, 153–65.CrossRefGoogle ScholarPubMed
Peters, W. (1970). Chemotherapy and Drug Resistance in Malaria. New York and London: Academic Press.Google Scholar
Ryley, J. F. & Betts, M. J. (1973). Chemotherapy of chicken coccidiosis. Advances in Pharmacology and Chemotherapy 11, 221–93.CrossRefGoogle ScholarPubMed
Schnitzer, R. J. (1963). Experimental chemotherapy. In Drug Resistance in Chemotherapy. A general outline, vol. 1 (ed. Schnitzer, R. J. and Hawking, F.), pp. 81–128. New York: Academic Press.Google Scholar