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Eimeria tenella glucose-6-phosphate isomerase: molecular characterization and assessment as a target for anti-coccidial control

Published online by Cambridge University Press:  17 March 2010

S.-S. LOO ,
D. P. BLAKE ,
A. MOHD-ADNAN ,
R. MOHAMED  and
K.-L. WAN
S.-S. LOO
Affiliation:
School of Biosciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor DE, Malaysia Malaysia Genome Institute, UKM-MTDC Technology Centre, 43600 UKM Bangi, Selangor DE, Malaysia
D. P. BLAKE
Affiliation:
Parasitology, Institute for Animal Health, Compton, Berkshire RG20 7NN, UK
A. MOHD-ADNAN
Affiliation:
School of Biosciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor DE, Malaysia Malaysia Genome Institute, UKM-MTDC Technology Centre, 43600 UKM Bangi, Selangor DE, Malaysia
R. MOHAMED
Affiliation:
School of Biosciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor DE, Malaysia Malaysia Genome Institute, UKM-MTDC Technology Centre, 43600 UKM Bangi, Selangor DE, Malaysia
K.-L. WAN*
Affiliation:
School of Biosciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor DE, Malaysia Malaysia Genome Institute, UKM-MTDC Technology Centre, 43600 UKM Bangi, Selangor DE, Malaysia
*
*Corresponding author: School of Biosciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor DE, Malaysia. Tel: +603 8921 5997. Fax: +603 8925 2698. E-mail: klwan@ukm.my
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Summary

Limitations with current chemotherapeutic and vaccinal control of coccidiosis caused by Eimeria species continue to prompt development of novel controls, including the identification of new drug targets. Glucose-6-phosphate isomerase (G6-PI) has been proposed as a valid drug target for many protozoa, although polymorphism revealed by electrophoretic enzyme mobility has raised doubts for Eimeria. In this study we identified and sequenced the Eimeria tenella G6-PI orthologue (EtG6-PI) from the reference Houghton strain and confirmed its position within the prevailing taxonomic hierarchy, branching with the Apicomplexa and Plantae, distinct from the Animalia including the host, Gallus gallus. Comparison of the deduced 1647 bp EtG6-PI coding sequence with the 9016 bp genomic locus revealed 15 exons, all of which obey the intron-AG-/exon/-GT-intron splicing rule. Comparison with the Weybridge and Wisconsin strains revealed the presence of 33 single nucleotide polymorphisms (SNPs) and 14 insertion/deletion sites. Three SNPs were exonic and all yielded non-synonymous substitutions. Preliminary structural predictions suggest little association between the coding SNPs and key G6-PI catalytic residues or residues thought to be involved in the coordination of the G6-PI's substrate phosphate group. Thus, the significant polymorphism from its host orthologue and minimal intra-specific polymorphism suggest G6-PI remains a valid anti-coccidial drug target.

Keywords

G6-PIcoccidiosisdrug targetEimeria tenella
Type
Research Article
Information
Parasitology , Volume 137 , Issue 8 , July 2010 , pp. 1169 - 1177
Copyright
Copyright © Cambridge University Press 2010

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