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Eimeria tenella: infection with a single sporocyst gives a clonal population

Published online by Cambridge University Press:  06 April 2009

M. W. Shirley  and
D. A. Harvey
M. W. Shirley*
Affiliation:
Institute for Animal Health, Compton Laboratory, Compton, Nr Newbury, Berks RG20 7NN
D. A. Harvey
Affiliation:
Institute for Animal Health, Compton Laboratory, Compton, Nr Newbury, Berks RG20 7NN
*
* Corresponding author. Tel: 01635 577275. Fax: 01635 577263. E-mail: shirley@bbsrc.ac.uk.
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Summary

Two genetically defined parents of Eimeria tenella, each characterized by a selectable phenotype, were mated to provide ‘recombinant’ oocysts for an analysis of the inheritance of DNA markers into the sporocyst stage of the life-cycle. A total of 23 populations was established from infection with a putative single sporocyst and their inheritance of DNA markers provided compelling evidence that the 2 sporozoites within each sporocyst are genetically identical. Each of the 5 polymorphic DNA markers used segregated independently within the ‘recombinant’ parasites and only 1 of the parental forms was inherited in 22 of the populations aimed to have been established from a single sporocyst.

Keywords

Eimeriacloningclonessporocysts
Type
Research Article
Information
Parasitology , Volume 112 , Issue 6 , June 1996 , pp. 523 - 528
Copyright
Copyright © Cambridge University Press 1996

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