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Enzymatic activities linked to cardiac energy metabolism of Trypanosoma evansi-infected rats and their possible functional correlations to disease pathogenesis

Published online by Cambridge University Press:  11 March 2015

MATHEUS D. BALDISSERA ,
VIRGINIA C. RECH ,
MATEUS GRINGS ,
LUCAS T. GRESSLER ,
RODRIGO A. VAUCHER ,
CLAITON I. SCHWERTZ ,
RICARDO E. MENDES ,
GUILHIAN LEIPNITZ ,
LENITA M. STEFANI  and
SILVIA G. MONTEIRO
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MATHEUS D. BALDISSERA*
Affiliation:
Department of Microbiology and Parasitology, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil
VIRGINIA C. RECH
Affiliation:
Laboratory of Nanotechnology, Centro Universitário Franciscano, Santa Maria, RS, Brazil
MATEUS GRINGS
Affiliation:
Department of Biochemistry, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil
LUCAS T. GRESSLER
Affiliation:
Department of Microbiology and Parasitology, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil
RODRIGO A. VAUCHER
Affiliation:
Laboratory of Microbiology, Centro Universitário Franciscano, Santa Maria, RS, Brazil
CLAITON I. SCHWERTZ
Affiliation:
Section of Veterinary Pathology, Instituto Federal Catarinense, Concórdia, SC, Brazil
RICARDO E. MENDES
Affiliation:
Section of Veterinary Pathology, Instituto Federal Catarinense, Concórdia, SC, Brazil
GUILHIAN LEIPNITZ
Affiliation:
Department of Biochemistry, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil
LENITA M. STEFANI
Affiliation:
Department of Animal Science, Universidade do Estado de Santa Catarina (UDESC), Chapecó, SC, Brazil
SILVIA G. MONTEIRO
Affiliation:
Department of Microbiology and Parasitology, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil
ALEKSANDRO S. DA SILVA*
Affiliation:
Department of Animal Science, Universidade do Estado de Santa Catarina (UDESC), Chapecó, SC, Brazil
*
* Corresponding author. Department of Microbiology and Parasitology, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil; Department of Animal Science, Universidade do Estado de Santa Catarina (UDESC), Chapecó, SC, Brazil. E-mail: matheusd.biomed@yahoo.com.br and aleksandro_ss@yahoo.com.br
* Corresponding author. Department of Microbiology and Parasitology, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil; Department of Animal Science, Universidade do Estado de Santa Catarina (UDESC), Chapecó, SC, Brazil. E-mail: matheusd.biomed@yahoo.com.br and aleksandro_ss@yahoo.com.br
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Summary

The aim of this study was to investigate the activities of important enzymes involved in the phosphoryl transfer network (adenylate kinase and creatine kinase (CK)), lactate dehydrogenase (LDH), respiratory chain complexes and biomarkers of cardiac function in rat experimentally infected by Trypanosoma evansi. Rat heart samples were evaluated at 5 and 15 days post-infection (PI). At 5 day PI, there was an increase in LDH and CK activities, and a decrease in respiratory chain complexes II, IV and succinate dehydrogenase activities. In addition, on day 15 PI, a decrease in the respiratory chain complex IV activity was observed. Biomarkers of cardiac function were higher in infected animals on days 5 and 15 PI. Considering the importance of the energy metabolism for heart function, it is possible that the changes in the enzymatic activities involved in the cardiac phosphotransfer network and the decrease in respiratory chain might be involved partially in the role of biomarkers of cardiac function of T. evansi-infected rats.

Keywords

Phosphotransfer networkcardiac muscle‘surra’respiratory chain activities
Type
Research Article
Information
Parasitology , Volume 142 , Issue 9 , August 2015 , pp. 1163 - 1170
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Copyright
Copyright © Cambridge University Press 2015 

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