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Evaluation of a monoclonal antibody-based competition ELISA for the diagnosis of human hydatidosis

Published online by Cambridge University Press:  06 April 2009

D. Liu ,
M. W. Lightowlers  and
M. D. Rickard
D. Liu
Affiliation:
University of Melbourne, Veterinary Clinical Centre, Princes Highway, Werribee, Victoria 3030, Australia
M. W. Lightowlers
Affiliation:
University of Melbourne, Veterinary Clinical Centre, Princes Highway, Werribee, Victoria 3030, Australia
M. D. Rickard
Affiliation:
C.S.I.R.O.Division of Animal Health, Private Bag No. 1, Parkville, Victoria 3052, Australia
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Summary

An antibody competition enzyme-linked immunosorbent assay (ELISA) using 4 different monoclonal antibodies (MAb) raised against major antigens (Antigen 5 and Antigen B) of Echinococcus granulosus was evaluated for the diagnosis of human hydatidosis. The competition assay, using anti-Ag5 MAb 24.14, detected specific antibodies in 70% (131/188) of sera from patients with surgically confirmed E. granulosus infection and 38·5% (10/26) of sera from patients with E. multilocularis infection. None of the sera from patients with Taenia solium cysticercosis (10), T. saginata (2), filariasis (22), strongyloidiasis (19), fascioliasis (4), bilharziasis (4) and amoebiasis (2) tested were positive using a cut-off point established through reaction between MAb 24.14 and normal human sera. The combined use of the MAb 24.14-based competition ELISA with the conventional antibody-binding assay provides a highly sensitive (92·8%) and specific screening system for human hydatid disease diagnosis.

Keywords

Echinococcus granulosusEchinococcus multilocularisTaenia soliumantibody competition ELISAimmuno-diagnosishydatidosisAntigen 5Antigen B
Type
Research Article
Information
Parasitology , Volume 104 , Issue 2 , April 1992 , pp. 357 - 361
Copyright
Copyright © Cambridge University Press 1992

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