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Evaluation of portable microscopic devices for the diagnosis of Schistosoma and soil-transmitted helminth infection

Published online by Cambridge University Press:  24 April 2014

ISAAC I. BOGOCH ,
JEAN T. COULIBALY ,
JASON R. ANDREWS ,
BENJAMIN SPEICH ,
JENNIFER KEISER ,
J. RUSSELL STOTHARD ,
ELIÉZER K. N'GORAN  and
JÜRG UTZINGER
ISAAC I. BOGOCH*
Affiliation:
Divisions of Internal Medicine and Infectious Diseases, Toronto General Hospital, 14EN-209, 200 Elizabeth Street, Toronto, ON, M5G 2C4, Canada
JEAN T. COULIBALY
Affiliation:
Unité de Formation et de Recherche Biosciences, Université Félix Houphouët-Boigny, 22 BP 770, Abidjan 22, Côte d'Ivoire Centre Suisse de Recherches Scientifiques en Côte d'Ivoire, 01 BP 1303, Abidjan 01, Côte d'Ivoire
JASON R. ANDREWS
Affiliation:
Division of Infectious Diseases and Geographic Medicine, Stanford University School of Medicine, Stanford, CA, 94305-5107, USA
BENJAMIN SPEICH
Affiliation:
Department of Medical Parasitology and Infection Biology, Swiss Tropical and Public Health Institute, P.O. Box, CH-4002 Basel, Switzerland University of Basel, P.O. Box, CH-4003 Basel, Switzerland
JENNIFER KEISER
Affiliation:
Department of Medical Parasitology and Infection Biology, Swiss Tropical and Public Health Institute, P.O. Box, CH-4002 Basel, Switzerland University of Basel, P.O. Box, CH-4003 Basel, Switzerland
J. RUSSELL STOTHARD
Affiliation:
Parasitology Department, Liverpool School of Tropical Medicine, Liverpool L3 5QA, UK
ELIÉZER K. N'GORAN
Affiliation:
Unité de Formation et de Recherche Biosciences, Université Félix Houphouët-Boigny, 22 BP 770, Abidjan 22, Côte d'Ivoire Centre Suisse de Recherches Scientifiques en Côte d'Ivoire, 01 BP 1303, Abidjan 01, Côte d'Ivoire
JÜRG UTZINGER
Affiliation:
University of Basel, P.O. Box, CH-4003 Basel, Switzerland Department of Epidemiology and Public Health, Swiss Tropical and Public Health Institute, P.O. Box, CH-4002 Basel, Switzerland
*
*Corresponding author. Divisions of Internal Medicine and Infectious Diseases, Toronto General Hospital, 14EN-209, 200 Elizabeth Street, Toronto, ON, M5G 2C4, Canada. E-mail: isaac.bogoch@uhn.ca
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Summary

The diagnosis of parasitic worm (helminth) infections requires specialized laboratory settings, but most affected individuals reside in locations without access to such facilities. We tested two portable microscopic devices for the diagnosis of helminth infections in a cross-sectional survey in rural Côte d'Ivoire. We examined 164 stool samples under a light microscope and then re-examined with a commercial portable light microscope and an experimental mobile phone microscope for the diagnosis of Schistosoma mansoni and soil-transmitted helminths. Additionally, 180 filtered urine samples were examined by standard microscopy and compared with the portable light microscope for detection of Schistosoma haematobium eggs. Conventional microscopy was considered the diagnostic reference standard. For S. mansoni, S. haematobium and Trichuris trichiura, the portable light microscope showed sensitivities of 84·8%, 78·6% and 81·5%, respectively, and specificities of 85·7%, 91·0% and 93·0%, respectively. For S. mansoni and T. trichiura, we found sensitivities for the mobile phone microscope of 68·2% and 30·8%, respectively, and specificities of 64·3% and 71·0%, respectively. We conclude that the portable light microscope has sufficient diagnostic yield for Schistosoma and T. trichiura infections, while the mobile phone microscope has only modest sensitivity in its current experimental set-up. Development of portable diagnostic technologies that can be used at point-of-sample collection will enhance diagnostic coverage in clinical and epidemiological settings.

Keywords

Diagnosismicroscopymobile phoneschistosomiasissoil-transmitted helminths
Type
Special Issue Article
Information
Parasitology , Volume 141 , Issue 14: Symposia of the British Society for Parasitology Volume 52 Advances in diagnostics for parasitic diseases , December 2014 , pp. 1811 - 1818
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Copyright
Copyright © Cambridge University Press 2014 

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