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Evidence for concanavalin A binding sites on the surface coat of Trypanosoma congolense

Published online by Cambridge University Press:  06 April 2009

P. Rautenberg ,
E. Reinwald  and
H-J. Risse
P. Rautenberg
Affiliation:
Institut für Veterinär-Biochemie, Freie Universität Berlin, Koserstrasse 20, 1000 Berlin 33, W. Germany
E. Reinwald
Affiliation:
Institut für Veterinär-Biochemie, Freie Universität Berlin, Koserstrasse 20, 1000 Berlin 33, W. Germany
H-J. Risse
Affiliation:
Institut für Veterinär-Biochemie, Freie Universität Berlin, Koserstrasse 20, 1000 Berlin 33, W. Germany
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Summary

Glycoproteins of Trypanosoma congolense have been detected on SDS-polyacrylamide gels using the Concanavalin A peroxidase technique. Using [35S]diazoniobenzenesulphonate as a marker for cell surface proteins it was possible to distinguish between internal glycoproteins and the surface coat proteins. On SDS-polyacrylamide gels Con A reacted with the surface coat proteins. Results obtained from Con A-induced agglutination of living trypanosomes indicated that sugars of the surface coat proteins were accessible to Con A. This was reinforced by the cytochemical visualization of Con A binding to the trypanosome surface. The results suggested that the surface coat protein contained α-linked d-mannosyl, d-glucosyl, or N-acetyl-d-glucosaminoyl residues, which are exposed exteriorly on the surface coat.

Type
Research Article
Information
Parasitology , Volume 80 , Issue 1 , February 1980 , pp. 113 - 122
Copyright
Copyright © Cambridge University Press 1980

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