Hostname: page-component-586b7cd67f-vdxz6 Total loading time: 0 Render date: 2024-11-20T16:15:28.407Z Has data issue: false hasContentIssue false
  • English
  • Français

Evidence for the absorption and synthesis of 5-hydroxytryptamine in perfused muscle and intestinal tissue and whole worms of adult Ascaris suum

Published online by Cambridge University Press:  06 April 2009

J. Chaudhuri ,
R. E. Martin  and
M. J. Donahue
J. Chaudhuri
Affiliation:
Department of Biological Sciences, Division of Cell and Molecular Biology, University of North Texas, Denton, Texas 76203USA
R. E. Martin
Affiliation:
Department of Biological Sciences, Division of Cell and Molecular Biology, University of North Texas, Denton, Texas 76203USA
M. J. Donahue
Affiliation:
Department of Biological Sciences, Division of Cell and Molecular Biology, University of North Texas, Denton, Texas 76203USA
Get access Rights & Permissions [Opens in a new window]

Summary

The metabolites of 5-hydroxytryptamine (5-HT, serotonin) namely, L-tryptophan, 5-hydroxytryptophan, 5-hydroxyindole acetic acid and 5-hydroxytryptophol were measured in perfused tissue and whole worms from adult female Ascaris suum using reversed-phase liquid chromatography with electrochemical detection. The intracellular levels of each metabolite were quantitated in response to several physiological effectors but only Ltryptophan (TRP) caused dose-dependent changes in these metabolites. Serotonin itself could also be absorbed by perfused A. suum muscle and intestinal tissue. When live A. suum were tied at the anterior and posterior regions to restrict TRP absorption by the intestine, TRP was absorbed through the cuticle and converted into 5-HT by the muscle tissue. In untied live parasites TRP absorption was observed in both muscle and intestinal tissue. Collectively, the data indicated that 5-HT may be either absorbed directly or synthesized de novo from absorbed TRP in the isolated tissue of A. suum. The 5-HT, in the live adult female A. suum, can be synthesized de novo from TRP, or 5-HT can be absorbed from the environment both through the cuticle and by the intestine of living parasites. Data also indicated that there was preferential sequestering of 5-HT and the metabolites of 5-HT in the anterior tissues of the worms.

Type
Research Article
Information
Parasitology , Volume 96 , Issue 1 , February 1988 , pp. 157 - 170
Copyright
Copyright © Cambridge University Press 1988

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Bennett, J. L. & Bueding, E. (1973). Uptake of 5-hydroxytryptamine by Schistosoma mansoni. Molecular Pharmacology 9, 311–19.Google ScholarPubMed
Donahue, M. J., Yacoub, N. J., Michnoff, C. A., Masaracchia, R. A. & Harris, B. G. (1981 a). Serotonin (5-hydroxytryptamino): a possible regulator of glycogenolysis in perfused muscle segments of Ascaris suum. Biochemical Biophysical Research Communications 101, 112–112.CrossRefGoogle ScholarPubMed
Donahue, M. J., Yacoub, N. J., Kaeini, M. R., Masaracchia, R. A. & Harris, B. G. (1981 b). Glycogen metabolizing enzymes during starvation and feeding of Ascaris suum maintained in a perfusion chamber. Journal of Parasilology 67, 505–505.CrossRefGoogle Scholar
Donahue, M. J., Yacoub, N. J. & Harris, B. G. (1982). Correlation of muscle activity with glycogen metabolism in muscle of Ascaris suum. American Journal of Physiology 242, R514–21.Google ScholarPubMed
Harpur, R. P. (1963). Maintenance of Ascaris lumbricoides in vitro. III. Changes in the hydrostatic skeleton. Comparative Biochemistry and Physiology 13, 71–85.CrossRefGoogle Scholar
Hsu, S.-C., Johansson, K. R. & Donahue, M. J. (1986). The bacterial flora of the intestine of Ascaris suum and 5-hydroxytryptamine production. Journal of Parasitology 72, 545–9.CrossRefGoogle ScholarPubMed
Kilts, C. D., Breese, G. R. & Mailman, R. B. (1981). Simultaneous quantification of dopamine, 5-hydroxytryptamine and four metabolically related compounds by means of reversed-phase high performance liquid chromatography with electrochemical detection. Journal of Chromatography 225, 347–57.CrossRefGoogle ScholarPubMed
Mansour, T. E. (1964). The pharmacology and biochemistry of parasitic helminths. Advances in Pharmacology 3, 217–23.Google ScholarPubMed
Mansour, T. E. (1979). Chemotherapy of parasitic worms: new biochemical strategies. Science 205, 462–9.CrossRefGoogle ScholarPubMed
Mansour, T. E. & Stone, D. B. (1970). Biochemical effects of lysergic acid diethylamide on the liver fluke Fasciola hepatica. Biochemical Pharmacology 19, 1137–46.CrossRefGoogle Scholar
Martin, R. E., Foster, L. A., Kester, A. S. & Donahue, M. J. (1987). Ascaris lumbricoides suum: morphological characterization of apparent cuticular pores by ionic permeability and electron microscopy. Experimental Parasitology 63, 329–36.CrossRefGoogle ScholarPubMed
Mettrick, D. F., Rahman, M. S. & Podesta, R. B. (1981). Effect of 5-hydroxytryptamine on in vitro glucose uptake and glycogen reserves in Hymenolepis diminuta. Molecular and Biochemical Parasitology 4, 217–23.CrossRefGoogle ScholarPubMed
Mishra, S. K., Sen, R. & Ghatak, T. (1983). Monoamine oxidase in adult Ascaridia galli. Journal of Helminthology 57, 313–18.CrossRefGoogle ScholarPubMed
Mishra, S. K., Sen, R. & Ghatak, T. (1984). Ascaris lumbricoides and Ascaridia galli: biogenic amines in adults and developmental stages. Experimental Parasitology 57, 34–9.CrossRefGoogle ScholarPubMed
Nimmo-Smith, R. H. & Raison, C. G. (1980). Monoamine oxidase activity of Schistosoma mansoni. Comparative Biochemistry and Physiology 24, 403–16.CrossRefGoogle Scholar
Ribeiro, P. & Webb, R. A. (1983). The synthesis of 5-hydroxytryptamine from tryptophan and 5-hydroxytryptophan in the cestode Hymenolepis diminuta. International Journal of Parasitology 13, 101–6.CrossRefGoogle ScholarPubMed
Ribeiro, P. & Webb, R. A. (1984). The occurrence, synthesis, and metabolism of 5-hydroxytryptamine and 5-hydroxytryptophan in the cestode Hymenolepis diminuta: a high performance liquid chromatographic study. Comparative Biochemistry and Physiology 79C, 159–64.Google Scholar
Sas, Statistical Analysis System Institute Inc. (1982). SAS User's Guide: Statistics SAS Institute Inc., Cary, N.C.Google Scholar
Wollenberger, A., Ristau, O. & Schuflag, G. (1960). A simple technique for extremely rapid freezing of large pieces of tissue. Pflügers Archiv für Anatomie und Physiologie 270, 399–412.CrossRefGoogle ScholarPubMed