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The evolution of metabolic profiling in parasitology

Published online by Cambridge University Press:  05 May 2010

E. HOLMES
E. HOLMES*
Affiliation:
Biomolecular Medicine, Department of Surgery and Cancer, Imperial College, London SW7 2AZ
*
Corresponding author: Tel.: +44(0)207 594 3220; Fax: +44(0)207 594 3226; E-mail: elaine.holmes@imperial.ac.uk
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Summary

The uses of metabolic profiling technologies such as mass spectrometry and nuclear magnetic resonance spectroscopy in parasitology have been multi-faceted. Traditional uses of spectroscopic platforms focused on determining the chemical composition of drugs or natural products used for treatment of parasitic infection. A natural progression of the use of these tools led to the generation of chemical profiles of the parasite in in vitro systems, monitoring the response of the parasite to chemotherapeutics, profiling metabolic consequences in the host organism and to deriving host-parasite interactions. With the dawn of the post-genomic era the paradigm in many research areas shifted towards Systems Biology and the integration of biomolecular interactions at the level of the gene, protein and metabolite. Although these technologies have yet to deliver their full potential, metabolic profiling has a key role to play in defining diagnostic or even prognostic metabolic signatures of parasitic infection and in deciphering the molecular mechanisms underpinning the development of parasite-induced pathologies. The strengths and weaknesses of the various spectroscopic technologies and analytical strategies are summarized here with respect to achieving these goals.

Keywords

Metabolic profilinghost-parasite interactionsspectroscopynetwork analysis
Type
Research Article
Information
Parasitology , Volume 137 , Special Issue 9: Insights into the metabolomes of parasites , August 2010 , pp. 1437 - 1449
Copyright
Copyright © Cambridge University Press 2010

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