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Expression of the histone chaperone SET/TAF-Iβ during the strobilation process of Mesocestoides corti (Platyhelminthes, Cestoda)

Published online by Cambridge University Press:  31 March 2015

CAROLINE B. COSTA ,
KARINA M. MONTEIRO ,
ALINE TEICHMANN ,
EDILEUZA D. DA SILVA ,
KARINA R. LORENZATTO ,
MARTÍN CANCELA ,
JÉSSICA A. PAES ,
ANDRÉ DE N. D. BENITZ ,
ESTELA CASTILLO  and
ROGÉRIO MARGIS
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CAROLINE B. COSTA
Affiliation:
Laboratório de Genômica Estrutural e Funcional, Laboratório de Biologia Molecular de Cestódeos, and Programa de Pós-Graduação em Biologia Celular e Molecular (PPGBCM), Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil
KARINA M. MONTEIRO
Affiliation:
Laboratório de Genômica Estrutural e Funcional, Laboratório de Biologia Molecular de Cestódeos, and Programa de Pós-Graduação em Biologia Celular e Molecular (PPGBCM), Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil Departamento de Biologia Molecular e Biotecnologia, Instituto de Biociências, UFRGS, Porto Alegre, RS, Brazil
ALINE TEICHMANN
Affiliation:
Laboratório de Genômica Estrutural e Funcional, Laboratório de Biologia Molecular de Cestódeos, and Programa de Pós-Graduação em Biologia Celular e Molecular (PPGBCM), Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil
EDILEUZA D. DA SILVA
Affiliation:
Laboratório de Genômica Estrutural e Funcional, Laboratório de Biologia Molecular de Cestódeos, and Programa de Pós-Graduação em Biologia Celular e Molecular (PPGBCM), Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil
KARINA R. LORENZATTO
Affiliation:
Laboratório de Genômica Estrutural e Funcional, Laboratório de Biologia Molecular de Cestódeos, and Programa de Pós-Graduação em Biologia Celular e Molecular (PPGBCM), Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil
MARTÍN CANCELA
Affiliation:
Laboratório de Genômica Estrutural e Funcional, Laboratório de Biologia Molecular de Cestódeos, and Programa de Pós-Graduação em Biologia Celular e Molecular (PPGBCM), Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil
JÉSSICA A. PAES
Affiliation:
Laboratório de Genômica Estrutural e Funcional, Laboratório de Biologia Molecular de Cestódeos, and Programa de Pós-Graduação em Biologia Celular e Molecular (PPGBCM), Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil
ANDRÉ DE N. D. BENITZ
Affiliation:
Laboratório de Genômica Estrutural e Funcional, Laboratório de Biologia Molecular de Cestódeos, and Programa de Pós-Graduação em Biologia Celular e Molecular (PPGBCM), Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil
ESTELA CASTILLO
Affiliation:
Sección Bioquímica y Biología Molecular, Facultad de Ciencias, Universidad de la República, Montevideo, Uruguay
ROGÉRIO MARGIS
Affiliation:
Laboratório de Genomas e Populações de Plantas, and PPGBCM, Centro de Biotecnologia, UFRGS, Porto Alegre, RS, Brazil
ARNALDO ZAHA
Affiliation:
Laboratório de Genômica Estrutural e Funcional, Laboratório de Biologia Molecular de Cestódeos, and Programa de Pós-Graduação em Biologia Celular e Molecular (PPGBCM), Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil Departamento de Biologia Molecular e Biotecnologia, Instituto de Biociências, UFRGS, Porto Alegre, RS, Brazil
HENRIQUE B. FERREIRA*
Affiliation:
Laboratório de Genômica Estrutural e Funcional, Laboratório de Biologia Molecular de Cestódeos, and Programa de Pós-Graduação em Biologia Celular e Molecular (PPGBCM), Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil Departamento de Biologia Molecular e Biotecnologia, Instituto de Biociências, UFRGS, Porto Alegre, RS, Brazil
*
* Corresponding author:LGEF, Centro de Biotecnologia, UFRGS, Caixa Postal 15005, 91501-970, Porto Alegre, RS, Brazil. E-mail: henrique@cbiot.ufrgs.br
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Summary

The histone chaperone SET/TAF-Iβ is implicated in processes of chromatin remodelling and gene expression regulation. It has been associated with the control of developmental processes, but little is known about its function in helminth parasites. In Mesocestoides corti, a partial cDNA sequence related to SET/TAF-Iβ was isolated in a screening for genes differentially expressed in larvae (tetrathyridia) and adult worms. Here, the full-length coding sequence of the M. corti SET/TAF-Iβ gene was analysed and the encoded protein (McSET/TAF) was compared with orthologous sequences, showing that McSET/TAF can be regarded as a SET/TAF-Iβ family member, with a typical nucleosome-assembly protein (NAP) domain and an acidic tail. The expression patterns of the McSET/TAF gene and protein were investigated during the strobilation process by RT-qPCR, using a set of five reference genes, and by immunoblot and immunofluorescence, using monospecific polyclonal antibodies. A gradual increase in McSET/TAF transcripts and McSET/TAF protein was observed upon development induction by trypsin, demonstrating McSET/TAF differential expression during strobilation. These results provided the first evidence for the involvement of a protein from the NAP family of epigenetic effectors in the regulation of cestode development.

Keywords

SET/TAF-IβMesocestoides corticestode developmentstrobilation process
Type
Research Article
Information
Parasitology , Volume 142 , Issue 9 , August 2015 , pp. 1171 - 1182
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Copyright
Copyright © Cambridge University Press 2015 

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