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Feeding of Boophilus microplus larvae on a partially defined medium through thin slices of cattle skin

Published online by Cambridge University Press:  06 April 2009

D. H. Kemp ,
D. Koudstaal ,
J. A. Roberts  and
J. D. Kerr
D. H. Kemp
Affiliation:
CSIRO, Long Pocket Laboratories, Division of Animal Health, Private Bag No. 3, P.O., Indooroopilly, Queensland 4068
D. Koudstaal
Affiliation:
CSIRO, Long Pocket Laboratories, Division of Animal Health, Private Bag No. 3, P.O., Indooroopilly, Queensland 4068
J. A. Roberts
Affiliation:
CSIRO, Long Pocket Laboratories, Division of Animal Health, Private Bag No. 3, P.O., Indooroopilly, Queensland 4068
J. D. Kerr
Affiliation:
CSIRO, Long Pocket Laboratories, Division of Animal Health, Private Bag No. 3, P.O., Indooroopilly, Queensland 4068
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Extract

Larvae of the cattle tick Boophilus microplus will attach to thin (0·3–0·5 mm) slices of cattle skin and engorge on a partially denned medium at 35 °C. Forty-seven to 83% of the larvae had engorged by 8 days, and 51–71% of these moulted to nymphs. Tissue culture medium alone allowed little growth unless supplemented with dialysed, freeze dried bovine serum (7%, w/v). This medium could be further defined by substituting purified bovine serum albumin (Cohn fraction V) for the dialysed bovine serum. In one experiment, nymphs of Haemaphysalis longicornis engorged and later moulted to adults. Skin slices were used fresh or after freeze-drying and storing at − 25 °C. The possible uses of the culture method are discussed.

Type
Research Article
Information
Parasitology , Volume 70 , Issue 2 , April 1975 , pp. 243 - 254
Copyright
Copyright © Cambridge University Press 1975

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