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A Gene associated with cell division and drug resistance in Giardia duodenalis

Published online by Cambridge University Press:  06 April 2009

J. A. Upcroft ,
A. Healey ,
D. G. Murray ,
P. F. L. Boreham  and
P. Upcroft
J. A. Upcroft
Affiliation:
Queensland Institute of Medical Research, The Bancroft Centre, 300 Herston Road, Brisbane QLD 4029, Australia
A. Healey
Affiliation:
Queensland Institute of Medical Research, The Bancroft Centre, 300 Herston Road, Brisbane QLD 4029, Australia
D. G. Murray
Affiliation:
Queensland Institute of Medical Research, The Bancroft Centre, 300 Herston Road, Brisbane QLD 4029, Australia
P. F. L. Boreham
Affiliation:
Queensland Institute of Medical Research, The Bancroft Centre, 300 Herston Road, Brisbane QLD 4029, Australia
P. Upcroft
Affiliation:
Queensland Institute of Medical Research, The Bancroft Centre, 300 Herston Road, Brisbane QLD 4029, Australia
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Extract

A 3000 bp cDNA insert (G6/1) from Giardia duodenalis, cloned into Escherichia coli is located on chromosome 3 of Giardia stocks which have 3 chromosomes detectable by field-inversion gel electrophoresis in the range 650–800 kb and on chromosome 3 and/or 4 of stocks with 4 chromosomes in this size range. The loss of this sequence from chromosome 4 but not chromosome 3 was associated with the induction of drug resistance in a previously sensitive laboratory stock. G6/1 appears to represent a single copy gene in Giardia as determined by hybridization of the probe to cleaved genomic DNA. Furthermore, the sequences flanking at least 12 kb of G6/1 are the same when G6/1 appears on both chromosomes 3 and 4. The cDNA encodes a protein associated with the nuclei of trophozoites during some stages of growth of the parasite. In a non-dividing culture, the antigen is associated with the nuclei of about 30% of trophozoites and fewer in a dividing culture. Three Giardia stocks with obvious chromosome rearrangements, which grow poorly and fail to divide normally, apparently lack the DNA sequence G6/1.

Keywords

Giardianuclear-associated antigendrug resistancechromosome rearrangementsgrowth mutantsstage-specific protein
Type
Research Article
Information
Parasitology , Volume 104 , Issue 3 , June 1992 , pp. 397 - 405
Copyright
Copyright © Cambridge University Press 1992

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