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Gene flow and cross-mating in Plasmodium falciparum in households in a Tanzanian village

Published online by Cambridge University Press:  06 April 2009

H. A. Babiker ,
J. D. Charlwood ,
T. Smith  and
D. Walliker
H. A. Babiker
Affiliation:
Institute of Cell, Animal and Population Biology, University of Edinburgh, West Mains Road, Edinburgh EH9 3JT, Scotland, UK
J. D. Charlwood
Affiliation:
National Institute for Medical Research, Ifakara Centre, P.O. Box 53, Ifakara, Tanzania
T. Smith
Affiliation:
Department of Public Health and Epidemiology, Swiss Tropical Institute, Postfach, CH-4002, Basel, Switzerland
D. Walliker
Affiliation:
Institute of Cell, Animal and Population Biology, University of Edinburgh, West Mains Road, Edinburgh EH9 3JT, Scotland, UK
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Summary

The diversity of the genes encoding 2 merozoite surface proteins (MSP-1 and MSP-2) of Plasmodium falciparum has been examined in parasites infecting members of 4 households in a village in Tanzania. The polymerase chain reaction (PCR) was used to characterize allelic variants of these genes by the sizes and sequences of regions of tandemly repeated bases in each gene. In each household extensive polymorphism was detected among parasites in the inhabitants and in infected mosquitoes caught in their houses. Similar frequencies of the alleles of these genes were observed in all households. Capture-recapture data indicated that both Anopheles gambiae and A.funestus freely dispersed among households in the hamlet. The results confirm that cross-mating and gene flow occur extensively among the parasites, and are discussed within the context of spatial clustering of natural populations of P. falciparum.

Keywords

Plasmodium falciparum,merozoite surface proteinsgenetic polymorphismsspatial clustering
Type
Research Article
Information
Parasitology , Volume 111 , Issue 4 , November 1995 , pp. 433 - 442
Copyright
Copyright © Cambridge University Press 1995

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