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Genetic and antigenic diversity of Theileria parva in cattle in Eastern and Southern zones of Tanzania. A study to support control of East Coast fever

Published online by Cambridge University Press:  24 November 2014

MWEGA ELISA ,
SALIH DIA HASAN ,
NJAHIRA MOSES ,
RUKAMBILE ELPIDIUS ,
ROBERT SKILTON  and
PAUL GWAKISA
MWEGA ELISA*
Affiliation:
Biosciences eastern and central Africa – International Livestock Research Institute Hub (BecA-ILRI Hub), P.O. Box 30709-00100, Nairobi, Kenya Faculty of Veterinary Medicine, Genome Science Center, Sokoine University of Agriculture (SUA), P.O. Box 3019, Morogoro, Tanzania
SALIH DIA HASAN
Affiliation:
Biosciences eastern and central Africa – International Livestock Research Institute Hub (BecA-ILRI Hub), P.O. Box 30709-00100, Nairobi, Kenya Veterinary Research Institute (VRI), P.O. Box 8067, Khartoum, Sudan
NJAHIRA MOSES
Affiliation:
Biosciences eastern and central Africa – International Livestock Research Institute Hub (BecA-ILRI Hub), P.O. Box 30709-00100, Nairobi, Kenya
RUKAMBILE ELPIDIUS
Affiliation:
Tanzania Veterinary Laboratory Agency (TVLA), P.O. Box 9254, Dar es Salaam, Tanzania
ROBERT SKILTON
Affiliation:
Biosciences eastern and central Africa – International Livestock Research Institute Hub (BecA-ILRI Hub), P.O. Box 30709-00100, Nairobi, Kenya
PAUL GWAKISA
Affiliation:
Faculty of Veterinary Medicine, Genome Science Center, Sokoine University of Agriculture (SUA), P.O. Box 3019, Morogoro, Tanzania
*
* Corresponding author: Mwega Elisa Biosciences eastern and central Africa – International Livestock Research Institute Hub (BecA-ILRI Hub), P.O. Box 30709-00100, Nairobi, Kenya. E-mail: elisa_mwega@yahoo.co.uk
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Summary

This study investigated the genetic and antigenic diversity of Theileria parva in cattle from the Eastern and Southern zones of Tanzania. Thirty-nine (62%) positive samples were genotyped using 14 mini- and microsatellite markers with coverage of all four T. parva chromosomes. Wright's F index (F ST = 0 × 094) indicated a high level of panmixis. Linkage equilibrium was observed in the two zones studied, suggesting existence of a panmyctic population. In addition, sequence analysis of CD8+ T-cell target antigen genes Tp1 revealed a single protein sequence in all samples analysed, which is also present in the T. parva Muguga strain, which is a component of the FAO1 vaccine. All Tp2 epitope sequences were identical to those in the T. parva Muguga strain, except for one variant of a Tp2 epitope, which is found in T. parva Kiambu 5 strain, also a component the FAO1 vaccine. Neighbour joining tree of the nucleotide sequences of Tp2 showed clustering according to geographical origin. Our results show low genetic and antigenic diversity of T. parva within the populations analysed. This has very important implications for the development of sustainable control measures for T. parva in Eastern and Southern zones of Tanzania, where East Coast fever is endemic.

Keywords

Cattlegenetic diversitymini-microsatellitesTanzaniaTheileria parva
Type
Research Article
Information
Parasitology , Volume 142 , Issue 5 , April 2015 , pp. 698 - 705
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Copyright
Copyright © Cambridge University Press 2014 

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