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Genetic characterization of parthenogenic Fasciola sp. in Japan on the basis of the sequences of ribosomal and mitochondrial DNA

Published online by Cambridge University Press:  11 July 2005

T. ITAGAKI ,
M. KIKAWA ,
K. SAKAGUCHI ,
J. SHIMO ,
K. TERASAKI ,
T. SHIBAHARA  and
K. FUKUDA
T. ITAGAKI
Affiliation:
Laboratory of Veterinary Parasitology, Faculty of Agriculture, Iwate University, Ueda 3-18-8, Morioka 020-8550, Japan
M. KIKAWA
Affiliation:
Laboratory of Veterinary Parasitology, Faculty of Agriculture, Iwate University, Ueda 3-18-8, Morioka 020-8550, Japan
K. SAKAGUCHI
Affiliation:
Laboratory of Veterinary Parasitology, Faculty of Agriculture, Iwate University, Ueda 3-18-8, Morioka 020-8550, Japan
J. SHIMO
Affiliation:
Laboratory of Veterinary Parasitology, Faculty of Agriculture, Iwate University, Ueda 3-18-8, Morioka 020-8550, Japan
K. TERASAKI
Affiliation:
St Mary's Junior College, Kurume 830-8558, Japan
T. SHIBAHARA
Affiliation:
Division of Laboratory Animal Science, Research Center for Bioscience and Technology, Tottori University, Yonago 683-8503, Japan
K. FUKUDA
Affiliation:
Center for Laboratory Animal Science, National Defense Medical College, Tokorozawa 359-8513, Japan
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Abstract

Accurate identification of aspermic Fasciola forms in Japan remains difficult because of their morphological variations. In order to characterize the forms genetically, nucleotide sequences of ribosomal internal transcribed spacer (ITS1 and ITS2) and mitochondrial cytochrome c oxidase I (COI) and NADH dehydrogenase I (NDI) genes in 34 liver flukes from 16 prefectures in Japan were analysed. Two major forms represented by Fsp 1 and Fsp 2 had sequences identical to or closely resembling those of F. hepatica and F. gigantica, respectively, in all the 4 DNA markers and were mainly distributed in northern and eastern-western parts of Japan, respectively. Fsp 1 and Fsp 2 would have been introduced into Japan with infected cattle of 2 distinct lineages via the Korean Peninsula and spread through limited parts of Japan (northern and eastern-western parts) together with the movement of each cattle lineage. The Japanese form (Fsp 1/2), which showed heterozygosity in ribosomal DNA and Fsp 2 haplotype in mitochondrial DNA, may have originated in interspecific cross hybridization between paternal F. hepatica and maternal F. gigantica.

Keywords

Fasciola spJapanparthenogenesishaplotypeITS1ITS2COINDIgenetic characterization
Type
Research Article
Information
Parasitology , Volume 131 , Issue 5 , November 2005 , pp. 679 - 685
Copyright
© 2005 Cambridge University Press

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