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Genetic control of resistance to infection with Nematospiroides dubius in mice: selection of high and low immune responder populations of mice

Published online by Cambridge University Press:  06 April 2009

P. Sitepu  and
C. Dobson
P. Sitepu
Affiliation:
Department of Parasitology, University of Queensland, St Lucia, Brisbane Qld 4067, Australia
C. Dobson
Affiliation:
Department of Parasitology, University of Queensland, St Lucia, Brisbane Qld 4067, Australia
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Summary

F1, Quackenbush (Q) × Wild (W) Mus musculus were assayed and selected for their level of immunity to secondary infections with 100 Nematospiroides dubius using faecal parasite egg counts. A significant positive linear correlation was found between the number of parasite eggs voided in the faeces and the number of N. dubius recovered after secondary infections in mice. Selection for immunity was not influenced by the conformation of the mice. High (H) and low (L) responder colonies together with a randomly (Rd) bred colony of mice were established over 5 generations. Adaptive immunity was more efficacious in protecting female than male mice and appeared to be controlled by a few dominant genes; the heritability (h2) of the trait was estimated at 0·56 for the parental stock and 0·55 for the F5 Rd colony and the realized heritability was 0·49. Higher anti-N. dubius antibody titres were detected in H than L mice. H mice harboured fewer and less fecund parasites than Rd colony mice; L mice had more worms with greater fecundities than Rd mice. The levels of primary infection in H and L mice related to the levels developed after secondary infection from F4. This suggested at least partial linkage of genes controlling innate with genes controlling adaptive immunity against N. dubius.

Type
Research Article
Information
Parasitology , Volume 85 , Issue 1 , August 1982 , pp. 73 - 84
Copyright
Copyright © Cambridge University Press 1982

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References

Ackert, J. E., Pratt, I. & Freeman, A. E. (1936). Resistant and susceptible groups of White Leghorn chickens to the nematode Ascaridea lineata (Scheider). Anatomical Record 67, 130.Google Scholar
Becker, W. A. (1968). Manual of Procedures in Quantitative Genetics. 2nd ed. Washington State University Pullman, Washington: Washington State University Press.Google Scholar
Behnke, J. M. & Wakelin, D. (1977). Nematospiroides dubius: Stimulation of acquired immunity in inbred strains of mice. Journal of Helminthology 51, 167–75.CrossRefGoogle ScholarPubMed
Brindley, P. J. & Dobson, C. (1981). Genetic control of liability to infection with Nematospiroides dubius in mice: selection of refractory and liable populations of mice. Parasitology 83, 51–65.CrossRefGoogle ScholarPubMed
Brindley, P. J. & Dobson, C. (1982). Multiple infections with Nematospiroides dubius in mice selected for liability to a single infection. Australian Journal of Experimental Biology and Medical Science (in the Press).CrossRefGoogle Scholar
Curtis, M. R., Dunning, W. F. & Bullock, F. D. (1933). Genetic factors in relation to the etiology of malignant tumors. American Journal of Cancer 17, 894–923.CrossRefGoogle Scholar
Cypess, R. H. & Zidian, J. L. (1975). Heligmosomoides polygrus (Nematospiroides dubius): The development of self-cure and/or protection in several strains of mice. Journal of Parasitology 61, 819–24.CrossRefGoogle ScholarPubMed
Dobson, C. (1982): Passive transfer of immunity with serum in mice infected with Nematospiroides dubius: influence of quality and quantity of immune serum. International Journal for Parasitology 12, 207–13.CrossRefGoogle ScholarPubMed
Dobson, C. & Owen, M. E. (1977). Influence of serial passage on the infectivity and immuno-genicity of Nematospiroides dubius in mice. International Journal for Parasitology 7, 463–6.CrossRefGoogle Scholar
Dobson, C. & Owen, M. E. (1978). Effect of host sex on passive immunity in mice infected with Nematospiroides dubius. International Journal for Parasitology 8, 359–64.CrossRefGoogle ScholarPubMed
Falconer, D. S. (1960). Introduction to Quantitative Genetics. New York: The Ronald Press Company.Google Scholar
Jenkins, D. C. & Carrington, T. S. (1981). Nematospiroides dubius: the course of primary, secondary and tertiary infections in high and low responder Biozzi mice. Parasitology 82, 311–18.CrossRefGoogle ScholarPubMed
Lueker, D. C. & Hepler, D. I. (1975). Differences in immunity to Nematospiroides dubius in inbred and outbred mice. Journal of Parasitology 61, 158–9.CrossRefGoogle ScholarPubMed
Ling, N. R. (1961). The attachment of proteins to aldehyde-tanned cells. British Journal of Haematology 7, 299–302.CrossRefGoogle ScholarPubMed
Prowse, S. J., Mitchell, G. F., Ey, P. L. & Jenkin, C. R. (1979). The development of resistance in different inbred strains of mice to infection with Nematospiroides dubius. Parasite Immunology 1, 277–88.CrossRefGoogle ScholarPubMed
Rubin, R., Lueker, D. C., Flom, J. O. & Anderssen, S. (1971). Immunity against Nematospiroides dubius in CFW Swiss Webster mice protected by subcutaneous larval vaccination. Journal of Parasitology 57, 815–17.CrossRefGoogle ScholarPubMed
Snedecor, G. W. & Cochran, W. G. (1968). Statistical Methods. 6th ed.Ames: The Iowa State University Press.Google Scholar
Van Zandt P. D. (1962). Immunity relationship in white mice infected with Nematospiroides dubius Baylis, 1926 (Nematode: Heligmosomidae). II. Artificial immunization with antigens prepared from larvae. Journal of Parasitology 47, 249–51.CrossRefGoogle Scholar
Wakelin, D. (1975). Genetic control of immune responses to parasites: selection for responsiveness and non-responsiveness to Trichuris muris in random-bred mice. Parasitology 71, 377–84.CrossRefGoogle ScholarPubMed
Wakelin, D. (1978). Genetic control of susceptibility and resistance to parasitic infection. Advances in Parasitology 16, 219–308.CrossRefGoogle ScholarPubMed
Webster, L. T. (1933). Inheritance and acquired factors in resistance to infection. I. Development of resistant and susceptible lines of mice through selective breeding. Journal of Experimental Medicine 57, 793–817.CrossRefGoogle ScholarPubMed