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Genetic divergence of human pathogens Nanophyetus spp. (Trematoda: Troglotrematidae) on the opposite sides of the Pacific Rim

Published online by Cambridge University Press:  01 December 2016

A. N. VORONOVA ,
G. N. CHELOMINA ,
V. V. BESPROZVANNYKH  and
V. V. TKACH
A. N. VORONOVA*
Affiliation:
Institute of Biology and Soil Science, Far Eastern Branch, Russian Academy of Sciences, 100–letiya Street, 159, Vladivostok 690022, Russia
G. N. CHELOMINA
Affiliation:
Institute of Biology and Soil Science, Far Eastern Branch, Russian Academy of Sciences, 100–letiya Street, 159, Vladivostok 690022, Russia Department of Biochemistry, Microbiology and Biotechnology, Far Eastern Federal University, Far Eastern Federal University, 690051 Vladivostok, Russia
V. V. BESPROZVANNYKH
Affiliation:
Institute of Biology and Soil Science, Far Eastern Branch, Russian Academy of Sciences, 100–letiya Street, 159, Vladivostok 690022, Russia
V. V. TKACH
Affiliation:
Department of Biology, University of North Dakota, 10 Grand Forks, ND 58202, USA
*
*Corresponding author. Institute of Biology and Soil Science, Far Eastern Branch, Russian Academy of Sciences, 100–letiya Street, 159, Vladivostok 690022, Russia. E-mail: avoronova92@gmail.com
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Summary

Human and animal nanophyetiasis is caused by intestinal flukes belonging to the genus Nanophyetus distributed on both North American and Eurasian coasts of Northern Pacific. In spite of the wide geographical distribution and medical and veterinary importance of these flukes, the intra-generic taxonomy of Nanophyetus spp. remains unresolved. The two most widely distributed nominal species, Nanophyetus salmincola and Nanophyetus schikhobalowi, both parasitizing humans and carnivorous mammals, were described from North America and eastern Eurasia, respectively. However, due to their high morphological similarity their interrelationships remained unclear and taxonomic status unstable. In this study, we explored genetic diversity of Nanophyetus spp. from the Southern Russian Far East in comparison with that of samples from North America based on the sequence variation of the nuclear ribosomal gene family (18S, internal transcribed spacers, ITS1-5·8S-ITS2 and 28S). High levels of genetic divergence in each rDNA region (nucleotide substitutions, indels, alterations in the secondary structures of the ITS1 and ITS2 transcripts) as well as results of phylogenetic analysis provided strong support for the status of N. salmincola and N. schikhobalowi as independent species.

Keywords

TrematodaNanophyetusITS18S rDNA28S rDNADNA secondary structuremolecular phylogenytaxonomy
Type
Research Article
Information
Parasitology , Volume 144 , Issue 5 , April 2017 , pp. 601 - 612
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Copyright
Copyright © Cambridge University Press 2016 

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