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Genetic recombination in Plasmodium berghei

Published online by Cambridge University Press:  06 April 2009

D. Walliker ,
R. Carter  and
S. Morgan
D. Walliker
Affiliation:
Protozoan Genetics Unit, Institute of Animal Genetics, West Mains Road, Edinburgh EH9 3JN
R. Carter
Affiliation:
Protozoan Genetics Unit, Institute of Animal Genetics, West Mains Road, Edinburgh EH9 3JN
S. Morgan
Affiliation:
Protozoan Genetics Unit, Institute of Animal Genetics, West Mains Road, Edinburgh EH9 3JN
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Extract

Crosses have been made between two lines of Plasmodium berghei yoelii differing in drug-sensitivity and enzyme-type. The two lines used were line A, which is pyrimethamine-resistant and contains an electrophoretic form of glucose phosphate isomerase termed GPI-1, and line C, which is pyrimethamine-sensitive and contains enzyme-form GPI-2. Equal numbers of blood forms of lines A and C were mixed and injected intravenously into a mouse. Mosquitoes were fed immediately on the mixture, and the resulting sporozoites used to infect further rodents. After treating these animals with pyrimethamine, drug-resistant parasites characterized by GPI-2 could be detected in the remaining infections. Controls showed that these parasites were recombinant forms, which had arisen by cross-fertilization of gametes in the mosquitoes, and not by mutation or ‘synpholia’. By cloning the products of crosses between lines A and C by dilution, parasite lines of two recombinant classes (resistant GPI-2 and. sensitive GPI-1) were isolated, together with lines of parental characteristics.

Type
Research Article
Information
Parasitology , Volume 66 , Issue 2 , April 1973 , pp. 309 - 320
Copyright
Copyright © Cambridge University Press 1973

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