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Genistein from Flemingia vestita (Fabaceae) enhances NO and its mediator (cGMP) production in a cestode parasite, Raillietina echinobothrida

Published online by Cambridge University Press:  24 April 2007

B. DAS ,
V. TANDON  and
N. SAHA
B. DAS
Affiliation:
Department of Zoology, North Eastern Hill University, Shillong-793022, India
V. TANDON*
Affiliation:
Department of Zoology, North Eastern Hill University, Shillong-793022, India
N. SAHA
Affiliation:
Department of Zoology, North Eastern Hill University, Shillong-793022, India
*
*Corresponding author. Tel: +91 364 2722312. Fax: +91 364 2550300/2722301. E-mail: tandonveena@hotmail.com
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Summary

Cyclic GMP (cGMP) is responsible for various cellular functions including signal pathways and it acts as a mediator for nitric oxide (NO). In order to evaluate the anthelmintic efficacy of the plant-derived isoflavones, the crude peel extract of Flemingia vestita and pure genistein were tested with respect to the activity of nitric oxide synthase (NOS), NO efflux and the cGMP concentration in Rallietina echinobothrida, the cestode parasite of domestic fowl. For comparison, the parasites were also treated with genistein (the major isoflavone present in the crude peel extract), sodium nitroprusside (SNP), a known NO donor, and praziquantel (PZQ), the reference drug. At the time of onset of paralysis in the parasite, the activity of NOS showed a significant increase (35–46%) and a 2-fold increase of NO efflux into the incubation medium in the treated worms in comparison to the respective controls. The cGMP concentration in the parasite tissue increased by 46–84% in the treated test worms in comparison to the controls. The results show that the isoflavones, genistein in particular, from the crude peel extract of F. vestita influence the cGMP concentration in the parasite tissue, which plays a major role in the downstream signal pathways.

Keywords

Flemingia vestitaRallietina echinobothridanitric oxide synthasenitric oxidecGMPgenistein
Type
Research Article
Information
Parasitology , Volume 134 , Issue 10 , September 2007 , pp. 1457 - 1463
Copyright
Copyright © Cambridge University Press 2007

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