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Giardia infection in pigs: detection and in vitro isolation of trophozoites of the Giardia intestinalis group

Published online by Cambridge University Press:  06 April 2009

B. Koudela ,
E. Nohýnková ,
J. Vítovec ,
M. Pakandl  and
J. Kulda
B. Koudela
Affiliation:
Department of Protozooses of Farm Animals, Institute of Parasitology, Czechoslovak Academy of Sciences, Branišovská 31, Ceské Budějovice 370 05, Czechoslovakia
E. Nohýnková
Affiliation:
Laboratory of Protozoology, Institute of Tropical Medicine, Sokolská 49, Prague 2, 120 00, Czechoslovakia
J. Vítovec
Affiliation:
Department of Protozooses of Farm Animals, Institute of Parasitology, Czechoslovak Academy of Sciences, Branišovská 31, Ceské Budějovice 370 05, Czechoslovakia
M. Pakandl
Affiliation:
Department of Protozooses of Farm Animals, Institute of Parasitology, Czechoslovak Academy of Sciences, Branišovská 31, Ceské Budějovice 370 05, Czechoslovakia
J. Kulda
Affiliation:
Department of Parasitology, Faculty of Science, Charles University, Viničná 7, Prague 2, 128 44, Czechoslovakia
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Summary

Giardia infections were detected in 14 out of 32 weaned, 1 to 2-month-old pigs, used in experiments with coccidia. Giardia trophozoites were present in the small intestine from the duodenum to the ileum with maximum numbers in the cranial part of the upper jejunum. They were localized mainly on the surface of intestinal crypts. No histological changes accompanied the infection and the infected animals were asymptomatic. Giardia trophozoites obtained from the intestines of necropsied pigs were isolated in axenic culture in a bile-supplemented TYI-S-33 medium. Morphological features of the trophozoites revealed by both light and scanning electron microscopy proved that the organism belongs to the Giardia intestinalis group.

Keywords

Giardia intestinalispigtrophozoite morphologyscanning electron microscopyaxenic culture
Type
Research Article
Information
Parasitology , Volume 102 , Issue 2 , April 1991 , pp. 163 - 166
Copyright
Copyright © Cambridge University Press 1991

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