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Gp63 gene polymorphism and population structure of Leishmania donovani complex: influence of the host selection pressure?

Published online by Cambridge University Press:  15 January 2002

S. GUERBOUJ ,
K. VICTOIR ,
I. GUIZANI ,
N. SERIDI ,
N. NUWAYRI-SALTI ,
M. BELKAID ,
R. BEN ISMAIL ,
D. Le RAY  and
J.C. DUJARDIN
S. GUERBOUJ
Affiliation:
Institut Pasteur de Tunis, Laboratoire d'Epidémiologie et Ecologie Parasitaire, 13 Place Pasteur BP74, 1002 Tunis Belvédère, Tunisia Prins Leopold Instituut voor Tropische Geneeskunde, Protozoology Unit, 155 Nationalestraat, B-2000 Antwerp, Belgium
K. VICTOIR
Affiliation:
Prins Leopold Instituut voor Tropische Geneeskunde, Protozoology Unit, 155 Nationalestraat, B-2000 Antwerp, Belgium
I. GUIZANI
Affiliation:
Institut Pasteur de Tunis, Laboratoire d'Epidémiologie et Ecologie Parasitaire, 13 Place Pasteur BP74, 1002 Tunis Belvédère, Tunisia
N. SERIDI
Affiliation:
Institut Pasteur d'Algérie, rue du Docteur Laveran, 16000 El Hamma, Algiers, Algeria
N. NUWAYRI-SALTI
Affiliation:
American University of Beirut, Bliss street, P.O. Box: 11-0236, Beirut, Lebanon
M. BELKAID
Affiliation:
Institut Pasteur d'Algérie, rue du Docteur Laveran, 16000 El Hamma, Algiers, Algeria
R. BEN ISMAIL
Affiliation:
Institut Pasteur de Tunis, Laboratoire d'Epidémiologie et Ecologie Parasitaire, 13 Place Pasteur BP74, 1002 Tunis Belvédère, Tunisia
D. Le RAY
Affiliation:
Prins Leopold Instituut voor Tropische Geneeskunde, Protozoology Unit, 155 Nationalestraat, B-2000 Antwerp, Belgium
J.C. DUJARDIN
Affiliation:
Prins Leopold Instituut voor Tropische Geneeskunde, Protozoology Unit, 155 Nationalestraat, B-2000 Antwerp, Belgium
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Abstract

The gp63 encoding genes were characterized by PCR–RFLP in 35 isolates representative of the Leishmania donovani complex (L. infantum, L. donovani, L. archibaldi and L. chagasi), with special attention to Mediterranean L. infantum from different geographical origins, and in separate groups from Old World Leishmania (L. major, L. tropica and L. aethiopica). The aim was to evaluate how the possible selective pressure by the host on these important surface proteins would influence structuring of our sample. Comparison was carried out with the structure obtained (i) from reported isoenzyme data, characters supposed to vary neutrally, and (ii) from PCR–RFLP analysis of gp63 inter-genic regions, containing non-translated spacers and regulatory genes. Polymorphism within the gp63-encoding region, was much higher than in gp63 inter-genic regions. In the gp63 intra-genic dendrogram, the 4 species of L. donovani complex were discriminated and quite distinct from outgroups. Within L. infantum, geographical structuring was observed and did not overlap with the structure built-up from isoenzymes and inter-genic data. These results support the idea of a strong host-selection on gp63, at vector level but most of all at vertebrate (human or dog) immunological level. Furthermore, they illustrate how the nature of genetic characters may influence the perception of population structuring.

Keywords

immunogenpolymorphismselectiondiagnosis
Type
Research article
Information
Parasitology , Volume 122 , Issue 1 , January 2001 , pp. 25 - 35
Copyright
© 2001 Cambridge University Press

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