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Heat shock and stress response of Taenia solium and T. crassiceps (Cestoda)

Published online by Cambridge University Press:  07 August 2001

L. VARGAS-PARADA ,
C. F. SOLÍS  and
J. P. LACLETTE
L. VARGAS-PARADA
Affiliation:
Department of Immunology, Instituto de Investigaciones Biomédicas, A.P. 70228, Cd. Universitaria 04510 México D.F., México
C. F. SOLÍS
Affiliation:
Department of Immunology, Instituto de Investigaciones Biomédicas, A.P. 70228, Cd. Universitaria 04510 México D.F., México
J. P. LACLETTE
Affiliation:
Department of Immunology, Instituto de Investigaciones Biomédicas, A.P. 70228, Cd. Universitaria 04510 México D.F., México
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Abstract

Heat shock and stress responses are documented for the first time in larval stages of the cestodes Taenia solium and Taenia crassiceps. Radioactive metabolic labelling after in vitro incubation of cysts at 43 °C, revealed the induction of heat shock proteins. In T. crassiceps, the major heat shock proteins were 80, 70 and 60 kDa. After prolonged incubation, a set of low molecular weight heat shock proteins (27, 31, 33 and 38 kDa), were also induced. In vitro incubation of cysts at 4 °C, induced the synthesis of stress proteins ranging from 31 to 80 kDa, indicating the parasite is also able to respond to cold shock. T. solium cysts exposure to temperature stress also resulted in an increased synthesis of 2 major heat shock proteins of 80 and 70 kDa. Western blots using the excretory–secretory products of T. solium showed that 2 heat shock proteins were recognized by antibodies in the sera of cysticercotic patients: one of 66 kDa and another migrating close to the run front. The T. solium 66 kDa protein was also recognized by specific antibodies directed to a 60 kDa bacterial heat shock protein, suggesting that it belongs to this family of proteins.

Keywords

Taenia soliumTaenia crassicepscestodesheat shock proteinsstressexcretory–secretory products
Type
Research Article
Information
Parasitology , Volume 122 , Issue 5 , May 2001 , pp. 583 - 588
Copyright
2001 Cambridge University Press

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