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High polymorphism in genes encoding antigen B from human infecting strains of Echinococcus granulosus

Published online by Cambridge University Press:  27 July 2005

L. KAMENETZKY ,
P. M. MUZULIN ,
A. M. GUTIERREZ ,
S. O. ANGEL ,
A. ZAHA ,
E. A. GUARNERA  and
M. C. ROSENZVIT
L. KAMENETZKY
Affiliation:
Departamento de Parasitología, Instituto Nacional de Enfermedades Infecciosas ANLIS “Dr. Carlos G. Malbrán” Av. Velez Sarsfield 563, Buenos Aires (1281), Argentina Instituto Tecnológico de Chascomús (IIB-INTECH)-Conicet/UNSAM, Chascomús, Argentina
P. M. MUZULIN
Affiliation:
Departamento de Parasitología, Instituto Nacional de Enfermedades Infecciosas ANLIS “Dr. Carlos G. Malbrán” Av. Velez Sarsfield 563, Buenos Aires (1281), Argentina
A. M. GUTIERREZ
Affiliation:
Departamento de Parasitología, Instituto Nacional de Enfermedades Infecciosas ANLIS “Dr. Carlos G. Malbrán” Av. Velez Sarsfield 563, Buenos Aires (1281), Argentina
S. O. ANGEL
Affiliation:
Instituto Tecnológico de Chascomús (IIB-INTECH)-Conicet/UNSAM, Chascomús, Argentina
A. ZAHA
Affiliation:
Centro de Biotecnologia e Departamento de Biologia Molecular e Biotecnologia, Instituto de Biociências, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil
E. A. GUARNERA
Affiliation:
Departamento de Parasitología, Instituto Nacional de Enfermedades Infecciosas ANLIS “Dr. Carlos G. Malbrán” Av. Velez Sarsfield 563, Buenos Aires (1281), Argentina
M. C. ROSENZVIT
Affiliation:
Departamento de Parasitología, Instituto Nacional de Enfermedades Infecciosas ANLIS “Dr. Carlos G. Malbrán” Av. Velez Sarsfield 563, Buenos Aires (1281), Argentina
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Abstract

Echinococcus granulosus antigen B (AgB) is encoded by a gene family and is involved in the evasion of the host immune response. E. granulosus exists as a number of strains (G1–G10) that differ in biological characteristics. We used PCR-SSCP followed by DNA sequencing to evaluate sequence variation and transcription profile of AgB in 5 E. granulosus strainsNucleotide sequence data reported in this paper are available in the GenBankTM database under the Accession numbers AY569341-AY569371, AY159484, AY159485, AY608606, AY608607, AY614001.. Twenty-four genomic sequences were isolated and clustered in 3 groups related to 2 of the 5 reported AgB genes. AgB4 genes were present in almost all strains, whereas AgB2 were present as functional genes exclusively in G1/G2 cluster, and as non-functional genes in G5 and the G6/G7 cluster, suggesting inter-strain variation. The AgB transcription patterns, analysed by RT-PCR, showed that AgB2 and AgB4 genes were transcribed in G1, while only the AgB4 gene was transcribed in G7 strain. Cysts from the same strain or cluster shared more genomic and cDNA variants than cysts from different strain or cluster. The level of nucleotide and deduced amino acid sequence variation observed is higher than that reported so far for coding genes of other helminths. Neutrality was rejected for AgB2 genes. These data show the genetic polymorphism of antigen-coding genes among genetically characterized strains of E. granulosus.

Keywords

genetic polymorphismantigen BEchinococcus granulosusstrainsRT-PCRSSCP
Type
Research Article
Information
Parasitology , Volume 131 , Issue 6 , December 2005 , pp. 805 - 815
Copyright
© 2005 Cambridge University Press

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