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Hymenolepis diminuta: metacestode-induced reduction in the synthesis of the yolk protein, vitellogenin, in the fat body of Tenebrio molitor

Published online by Cambridge University Press:  06 April 2009

T. J. Webb  and
H. Hurd
T. J. Webb*
Affiliation:
Centre for Applied Entomology and Parasitology, Department of Biological Sciences, Keele University, Keele, Staffs ST5 5BG, UK
H. Hurd
Affiliation:
Centre for Applied Entomology and Parasitology, Department of Biological Sciences, Keele University, Keele, Staffs ST5 5BG, UK
*
* Corresponding author Dr Tracey Webb.
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Summary

Vitellogenin synthesis by the fat body has been monitored using in vitro culture and immunoprecipitation. This system was found to be efficient for measuring vitellogenin production in both non-infected Tenebrio molitor and those infected with Hymenolepis diminuta. In fat bodies from infected beetles, vitellogenin production was decreased by up to 75% (day 24 post-infection) and, at all times investigated, vitellogenin synthesis was significantly below control levels (days 3–30 post-infection). Incubating fat bodies from control insects with isolated metacestodes indicated that this may be a direct effect by the parasite which is developmental stage-specific. Stage II, but not stage III–IV, nor heat-killed parasites could bring about this decrease in vitellogenin. In addition, these effects may be density dependent within the range of 2–20 parasites per fat body; only 2 metacestodes were necessary to cause a significant decrease. Since metacestodes do not take up vitellogenin, nor limit the amount of [14C] leucine available to the fat body for vitellogenin production, it is conceivable that the parasite produces a potent inhibitor of vitellogenin synthesis, or a molecule which induces cells within the fat body to do so.

Keywords

Tenebrio molitorHymenolepis diminutaCestodavitellogeninvitellognesisfat body
Type
Research Article
Information
Parasitology , Volume 112 , Issue 4 , April 1996 , pp. 429 - 436
Copyright
Copyright © Cambridge University Press 1996

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