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Identification of ABC transporters in Sarcoptes scabiei

Published online by Cambridge University Press:  03 February 2006

K. E. MOUNSEY ,
D. C. HOLT ,
J. McCARTHY  and
S. F. WALTON
K. E. MOUNSEY
Affiliation:
Menzies School of Health Research, Darwin NT Australia Institute of Advanced Studies, Charles Darwin University, Darwin NT Australia
D. C. HOLT
Affiliation:
Menzies School of Health Research, Darwin NT Australia Institute of Advanced Studies, Charles Darwin University, Darwin NT Australia
J. McCARTHY
Affiliation:
Queensland Institute of Medical Research, University of Queensland, Brisbane, QLD Australia
S. F. WALTON
Affiliation:
Menzies School of Health Research, Darwin NT Australia Institute of Advanced Studies, Charles Darwin University, Darwin NT Australia
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Abstract

We have identified and partially sequenced 8 ABC transporters from an EST dataset of Sarcoptes scabiei var. hominis, the causative agent of scabies. Analysis confirmed that most of the known ABC subfamilies are represented in the EST dataset including several members of the multidrug resistance protein subfamily (ABC-C). Although P-glycoprotein (ABC-B) sequences were not found in the EST dataset, a partial P-glycoprotein sequence was subsequently obtained using a degenerate PCR strategy and library screening. Thus a total of 9 potential S. scabiei ABC transporters representing the subfamilies A, B, C, E, F and H have been identified. Ivermectin is currently used in the treatment of hyper-infested (crusted) scabies, and has also been identified as a potentially effective acaricide for mass treatment programmes in scabies-endemic communities. The observation of clinical and in vitro ivermectin resistance in 2 crusted scabies patients who received multiple treatments has raised serious concerns regarding the sustainability of such programmes. One possible mechanism for ivermectin resistance is through ABC transporters such as P-glycoprotein. This work forms an important foundation for further studies to elucidate the potential role of ABC transporters in ivermectin resistance of S. scabiei.

Keywords

Sarcoptes scabieiivermectin resistanceABC transporter
Type
Research Article
Information
Parasitology , Volume 132 , Issue 6 , June 2006 , pp. 883 - 892
Copyright
2006 Cambridge University Press

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