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Immune cell arrival kinetics to peritoneum and role during murine-experimental trichomoniasis

Published online by Cambridge University Press:  09 August 2021

F. J. Rangel-Mata ,
E. E. Ávila-Muro Open the ORCID record for E. E. Ávila-Muro [Opens in a new window] ,
J. E. Reyes-Martínez Open the ORCID record for J. E. Reyes-Martínez [Opens in a new window] ,
L. M. Olmos-Ortiz Open the ORCID record for L. M. Olmos-Ortiz [Opens in a new window] ,
M. E. Brunck ,
L. A. Arriaga-Pizano  and
P. Cuéllar-Mata Open the ORCID record for P. Cuéllar-Mata [Opens in a new window]
F. J. Rangel-Mata
Affiliation:
Departamento de Biología, Universidad de Guanajuato, Guanajuato, Mexico
E. E. Ávila-Muro
Affiliation:
Departamento de Biología, Universidad de Guanajuato, Guanajuato, Mexico
J. E. Reyes-Martínez
Affiliation:
Departamento de Biología, Universidad de Guanajuato, Guanajuato, Mexico
L. M. Olmos-Ortiz
Affiliation:
Departamento de Biología, Universidad de Guanajuato, Guanajuato, Mexico
M. E. Brunck
Affiliation:
Escuela de Ingeniería y Ciencias, Tecnológico de Monterrey, Monterrey, Mexico
L. A. Arriaga-Pizano
Affiliation:
Unidad de Investigación Médica en Inmunoquímica, IMMS, Ciudad de México, Mexico
P. Cuéllar-Mata*
Affiliation:
Departamento de Biología, Universidad de Guanajuato, Guanajuato, Mexico
*
Author for correspondence: P. Cuéllar-Mata, E-mail: mata@ugto.mx
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Abstract

Trichomonas vaginalis causes trichomoniasis, an inflammatory process related to an increased rate of HIV transmission. In order to study T. vaginalis infection response in a microorganism-free environment, an infection model was established providing a host–parasite interaction system useful to study the interplay between immune cells and the parasite. Infected mice peritoneal cells were immunophenotyped at different times after infection using flow cytometry. Neutrophils and macrophages showed the most relevant increase from third to 12th day post-infection. A high number of B lymphocytes were present on 15th day post-infection, and an increase in memory T cells was observed on sixth day post-infection. The levels of NO increased at day 10 post-infection; no significant influence was observed on T. vaginalis clearance. Increased viability of T. vaginalis was observed when the NETs inhibitors, metformin and Cl− amidine, were administrated, highlighting the importance of this mechanism to control parasite infection (43 and 86%, respectively). This report presents a comprehensive cell count of the immune cells participating against trichomoniasis in an in vivo interaction system. These data highlight the relevance of innate mechanisms such as specific population changes of innate immune cells and their impact on the T. vaginalis viability.

Keywords

Host–parasite interactionNETstrichomoniasis
Type
Research Article
Information
Parasitology , Volume 148 , Issue 13 , November 2021 , pp. 1624 - 1635
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Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press

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