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Immunization of pigs against infection with Schistosoma japonicum using ultraviolet-attenuated cercariae

Published online by Cambridge University Press:  06 April 2009

Y.-E. Shi ,
C.-F. Jiang ,
J.-J. Han ,
Y.-L. Li  and
A. Ruppel
Y.-E. Shi
Affiliation:
Department of Parasitology, Tongji Medical University, Hankong Lu 13, Wuhan, 430030, People's Republic of China
C.-F. Jiang
Affiliation:
Department of Parasitology, Tongji Medical University, Hankong Lu 13, Wuhan, 430030, People's Republic of China
J.-J. Han
Affiliation:
Department of Parasitology, Tongji Medical University, Hankong Lu 13, Wuhan, 430030, People's Republic of China
Y.-L. Li
Affiliation:
Department of Parasitology, Tongji Medical University, Hankong Lu 13, Wuhan, 430030, People's Republic of China
A. Ruppel
Affiliation:
Institute for Tropical Hygiene, Im Neuenheimer Feld 324, D-6900 Heidelberg, Germany
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Summary

Since pigs are important in the zoonotic transmission of schistosomiasis japonica in China, a veterinary vaccine might contribute to the control of the disease in humans. Pigs were immunized with three doses each of 10000 cercariae of Schistosoma japonicum attenuated with ultraviolet light (400 μWatt·min/cm2). The experiment was performed with portable irradiation equipment in a rural area of the Hubei Province (P.R. China). A challenge infection of 1000 untreated cercariae was given 2·5 or 6 months after the last immunization, and age-matched naive pigs were challenged as a control. Immunized pigs developed about 90% resistance against the challenge. The liver egg load of these animals was reduced by over 90%. Less than 0·01 % of the immunizing cercariae developed to adult parasites and the vaccination had no apparent adverse influence on the pigs' health.

Keywords

Schistosoma japonicumpigsvaccinationultraviolet lightattenuationcercariae
Type
Research Article
Information
Parasitology , Volume 106 , Issue 5 , June 1993 , pp. 459 - 462
Copyright
Copyright © Cambridge University Press 1993

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References

REFERENCES

Chao, W. X. (1983). Human Parasitology, pp. 327–8. Beijing: People's Health Publishing House (in Chinese).Google Scholar
Committee for Research on Schistosomiasis, Hubei (1991). A Corpus of Studies on Schistosomiasis japonica 1989–1990, p. 7 (in Chinese).Google Scholar
Dean, D. A., Bukowski, M. A. & Clark, S. S. (1981). Attempts to transfer the resistance of Schistosoma mansoni-infected and irradiated cercariae-immunized mice by means of parabiosis. American Journal of Tropical Medicine and Hygiene 30, 113–20.CrossRefGoogle Scholar
Ho, Y. H. (1963). On the host specificity of Schistosoma japonicum. Chinese Medical Journal 82, 403–14.Google ScholarPubMed
Hsü, S. Y. L., Hsü, H. F., Xu, S. T., Shi, F. H., He, Y. X., Clarke, W. R. & Johnson, S. C. (1983). Vaccination against bovine schistosomiasis japonica with highly X-irradiated schistosomula. American Journal of Tropical Medicine and Hygiene 29, 367–70.CrossRefGoogle Scholar
Hsü, S. Y. L., Xu, S. T., He, Y. X., Shi, F. H., Shen, W., Hsü, H. F., Osborne, J. W. & Clarke, W. R. (1984). Vaccination of bovines against schistosomiasis japonica with highly irradiated schistosomula in China. American Journal of Tropical Medicine and Hygiene 33, 891–8.CrossRefGoogle ScholarPubMed
Mao, S. p. (1990). Biology of Schistosoma and Control of Schistosomiasis, p. 630. Beijing: People's Health Publishing House (in Chinese).Google Scholar
Moloney, N. A., Bickle, Q. D. & Webbe, G. (1985). The induction of specific immunity against Schistosoma japonicum by exposure of mice to ultraviolet attenuated cercariae. Parasitology 90, 313–23.CrossRefGoogle ScholarPubMed
Moloney, N. A., Hinchcliffe, P. & Webbe, G. (1987 a). Loss of resistance to reinfection with Schistosoma japonicum in mice after treatment with praziquantel. Transactions of the Royal Society of Tropical Medicine and Hygiene 81, 247–54.CrossRefGoogle ScholarPubMed
Moloney, N. A., Webbe, G. & Hinchcliffe, P. (1987 b). The induction of species-specific immunity against Schistosoma japonicum by exposure of rats to ultra-violet attenuated cercariae. Parasitology 94, 49–54.CrossRefGoogle ScholarPubMed
Ruppel, A., Shi, Y. E. & Moloney, N. A. (1990). Schistosoma mansoni and S. japonicum: comparison of levels of ultraviolet irradiation for vaccination of mice with cercariae. Parasitology 100, 23–6.CrossRefGoogle Scholar
Shi, Y. E., Jiang, C. F., Han, J. J., Li, Y. L. & Ruppel, A. (1990). Schistosoma japonicum: an ultraviolet- attenuated vaccine applicable in the field for water buffaloes. Experimental Parasitology 71, 100–6.CrossRefGoogle ScholarPubMed
Taylor, M. G. (1987). Schistosomes of domestic animals: Schistosoma bovis and other animal forms. In Immune Responses in Parasitic Infections: Immunology, Immunopathology and Immunoprophylaxis, vol. 2 (ed. Soulsby, E. J. L.), pp. 49–90. Boca Raton, Florida: CRC Press.Google Scholar
Wan, Z. G. & Gao, W. C. (1986). Advances in the epidemiology of schistosomiasis in China. Jiangsu Medical Journal, 60–3 (in Chinese).Google Scholar
Zhu, B. (1990). Victories in snail fever battle. China Daily 10, no. 2900, 1.Google Scholar