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Immunogenetic correlates of susceptibility to infection with Heligmosomoides polygyrus in outbred mice

Published online by Cambridge University Press:  06 April 2009

A. E. Keymer ,
A. B. Tarlton ,
R. W. Hiorns ,
C. E. Lawrence  and
D. I. Pritchard
A. E. Keymer
Affiliation:
Department of Zoology, University of Oxford, South Parks Road, Oxford OX1 3PS
A. B. Tarlton
Affiliation:
Department of Zoology, University of Oxford, South Parks Road, Oxford OX1 3PS
R. W. Hiorns
Affiliation:
Department of Zoology, University of Oxford, South Parks Road, Oxford OX1 3PS
C. E. Lawrence
Affiliation:
Department of Zoology, University of Oxford, South Parks Road, Oxford OX1 3PS
D. I. Pritchard
Affiliation:
Department of Zoology, University of Oxford, South Parks Road, Oxford OX1 3PS
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Summary

Outbred MF1 mice were characterized with respect to their susceptibility to infection with Heligmosomoides polygyrus (Nematoda) on the basis of faecal egg counts after 8 weeks of repeated infection (50 larvae/week). Selective breeding for resistance and susceptibility was carried out over 3 generations. The H-2 type of a sample of the mice was determined, and antigen recognition assessed on the basis of Western blots against adult and larval H. polygyrus homogenate. The selective breeding programme yielded very strong evidence for the heritability of susceptibility to infection. The results were consistent with a model of single gene control with resistance dominant over susceptibility. The presence of the H-2k haplotype was significantly associated with susceptibility, as was the recognition of a 17 kDa antigen in blots against both larval and adult worm homogenate. The proportion of mice phenotypically susceptible to infection, the proportion bearing the H-2k haplotype, and the proportion recognizing the 17 kDa antigen, were all approximately 0.25.

Keywords

Heligomosomoides polygyrussusceptibilityH-2 typesoutbred mice
Type
Research Article
Information
Parasitology , Volume 101 , Issue 1 , August 1990 , pp. 69 - 73
Copyright
Copyright © Cambridge University Press 1990

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