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Immunoglobulin-G binding proteins in the ixodid ticks, Rhipicephalus appendiculatus, Amblyomma variegatum and Ixodes hexagonus

Published online by Cambridge University Press:  06 April 2009

H. Wang  and
P. A. Nuttall
H. Wang
Affiliation:
NERC Institute of Virology and Environmental Microbiology, Mansfield Road, Oxford OX1 3SR
P. A. Nuttall
Affiliation:
NERC Institute of Virology and Environmental Microbiology, Mansfield Road, Oxford OX1 3SR
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Summary

A guinea-pig immunoglobulin-G linked agarose column was used to detect the immunoglobulin-G binding proteins (IGBP) in the ixodid ticks, Rhipicephalus appendiculatus, Amblyomma variegatum and Ixodes hexagonus. IGBPs were found in salivary gland extract (SGE) of the unfed ticks of all 3 species. The sizes of the IGBPs, as determined by SDS–polyacrylamide gel electrophoresis, differed between species. In R. appendiculatus 2 IGBP bands were detected in haemolymph collected from unfed females and males, and an additional 78 kDa IGBP was detected in haemolymph samples of male and female ticks that had fed for 6 days. Although there was no difference in the IGBP profile of SGE derived from unfed male and female R. appendiculatus, 2 new IGBPs were detected in the SGE of the male ticks after they had fed for 6 days. The possible function of IGBP in ticks is discussed.

Keywords

immunoglobulin binding proteintick salivary glandtick haemolymphRhipicephalus appendiculatusAmblyomma variegatumIxodes hexagonus
Type
Research Article
Information
Parasitology , Volume 111 , Issue 2 , August 1995 , pp. 161 - 165
Copyright
Copyright © Cambridge University Press 1995

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