Hostname: page-component-cd9895bd7-fscjk Total loading time: 0 Render date: 2024-12-27T23:02:02.946Z Has data issue: false hasContentIssue false
  • English
  • Français

Immunolocalization of the fatty acid-binding protein Sj-FABPc within adult Schistosoma japonicum

Published online by Cambridge University Press:  01 July 1997

G. N. GOBERT ,
D. J. STENZEL ,
M. K. JONES  and
D. P. McMANUS
G. N. GOBERT
Affiliation:
Analytical Electron Microscopy Facility, Queensland University of Technology, GPO Box 2434, George Street, Brisbane, Queensland 4001, Australia
D. J. STENZEL
Affiliation:
Analytical Electron Microscopy Facility, Queensland University of Technology, GPO Box 2434, George Street, Brisbane, Queensland 4001, Australia
M. K. JONES
Affiliation:
Centre for Microscopy and Microanalysis, The University of Queensland, St Lucia, Brisbane, Queensland 4072, Australia
D. P. McMANUS
Affiliation:
Australian Centre for International and Tropical Health and Nutrition, Queensland Institute of Medical Research, 300 Herston Road, Herston, Brisbane, Queensland 4029, Australia
Get access Rights & Permissions [Opens in a new window]

Abstract

This paper describes the first localization study of the 14·7 kDa fatty acid-binding protein in Schistosoma japonicum (Sj-FABPc) using transmission electron microscopy. A polyclonal antibody raised against recombinant Sj-FABPc was used in combination with a colloidal gold marker to determine the distribution of the protein within adult parasites. Sj-FABPc was localized within lipid droplets below the subtegumental region of the male parasite. Additionally, Sj-FABPc was present in the vitelline droplets of the vitelline glands of female parasites. There were no detectable levels of Sj-FABPc on the surface or within the tegument of male or female parasites. Possible functions of Sj-FABPc within S. japonicum and the relevance of these immunolocalization findings in light of the recent reports that the homologue Sm-FABPc is an important anti-S. mansoni vaccine target molecule are also discussed.

Keywords

Schistosoma japonicumFABPcelectron microscopyimmunolocalizationcryopreparationvitelline glands
Type
Research Article
Information
Parasitology , Volume 115 , Issue 1 , July 1997 , pp. 33 - 39
Copyright
1997 Cambridge University Press

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)