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Impact of collaborative actions of Leishmania (Viannia) braziliensis subpopulations on the infection profile

Published online by Cambridge University Press:  13 July 2022

Barbara Cristina de Albuquerque-Melo Open the ORCID record for Barbara Cristina de Albuquerque-Melo [Opens in a new window] ,
Léa Cysne-Finkelstein Open the ORCID record for Léa Cysne-Finkelstein [Opens in a new window] ,
Luiz Filipe Gonçalves-Oliveira ,
Cynthia Machado Cascabulho Open the ORCID record for Cynthia Machado Cascabulho [Opens in a new window] ,
Andrea Henriques-Pons Open the ORCID record for Andrea Henriques-Pons [Opens in a new window] ,
Mirian Cláudia de Souza Pereira Open the ORCID record for Mirian Cláudia de Souza Pereira [Opens in a new window] ,
Carlos Roberto Alves Open the ORCID record for Carlos Roberto Alves [Opens in a new window]  and
Geovane Dias-Lopes Open the ORCID record for Geovane Dias-Lopes [Opens in a new window]
Barbara Cristina de Albuquerque-Melo
Affiliation:
Laboratório de Biologia Molecular e Doenças Endêmicas, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, Brasil
Léa Cysne-Finkelstein
Affiliation:
Laboratório de Imunoparasitologia, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, Brasil
Luiz Filipe Gonçalves-Oliveira
Affiliation:
Laboratório de Biologia Molecular e Doenças Endêmicas, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, Brasil
Cynthia Machado Cascabulho
Affiliation:
Laboratório de Inovação em Terapias, Ensino e Bioprodutos, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, Brasil
Andrea Henriques-Pons
Affiliation:
Laboratório de Inovação em Terapias, Ensino e Bioprodutos, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, Brasil
Mirian Cláudia de Souza Pereira
Affiliation:
Laboratório de Ultraestrutura Celular, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, Brasil
Carlos Roberto Alves*
Affiliation:
Laboratório de Biologia Molecular e Doenças Endêmicas, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, Brasil
Geovane Dias-Lopes
Affiliation:
Laboratório de Biologia Molecular e Doenças Endêmicas, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, Brasil
*
Author for correspondence: Carlos Roberto Alves, E-mail: calves@ioc.fiocruz.br
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Abstract

This study focuses on the role of the population structure of Leishmania spp. on the adaptive capacity of the parasite. Herein, we investigate the contribution of subpopulations of the L. (V.) braziliensis Thor strain (Thor03, Thor10 and Thor22) in the profile of murine macrophages infection. Infection assays were performed with binary combinations of these subpopulations at stationary phases. The initial interaction time showed major effects on the combination assays, as demonstrated by the significant increase in the infection rate at 5 h. Based on the endocytic index (EI), Thor10 (EI = 563.6) and Thor03 (EI = 497) showed a higher infection load compared to Thor22 (EI = 227.3). However, the EI decreased in Thor03 after 48 h (EI = 447) and 72 h (EI = 388.3) of infection, and showed changes in the infection level in all Thor10/Thor22 combinations. Assays with CellTrace CFSE-labelled Thor22 promastigotes indicated an increase (~1.5 fold) in infection by this subpopulation in the presence of Thor10 when compared to the infection profile of Thor03/Thor22 combinations in the same proportions. In addition, the potential of these subpopulations, alone or in binary combinations, to modulate the expression of cytokines and nitric oxide (NO) in vitro was investigated. Lower NO and tumour necrosis factor-α production levels were observed for all Thor10/Thor22 combinations at 24 h compared to these subpopulations alone. In contrast, Thor03/Thor22 combination assays increased IL-10 production at this time. Collectively, these results provide in vitro evidence on the potential of L. (V.) braziliensis population structure to play a relevant role in a host infection by this parasite.

Keywords

CFSE labellingcytokinesin vitro infectionLeishmania (Viannia) braziliensismurine peritoneal macrophagespopulation structure
Type
Research Article
Information
Parasitology , Volume 149 , Issue 12 , October 2022 , pp. 1526 - 1535
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Copyright
Copyright © The Author(s), 2022. Published by Cambridge University Press

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