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Impact of temporal changes and host factors on the genetic structure of a population of Opisthorchis viverrini sensu lato in Khon Kaen Province (Thailand)

Published online by Cambridge University Press:  15 June 2009

W. SAIJUNTHA ,
P. SITHITHAWORN ,
N. B. CHILTON ,
T. N. PETNEY ,
S. KLINBUNGA ,
R. SATRAWAHA ,
J. P. WEBSTER  and
R. H. ANDREWS
W. SAIJUNTHA
Affiliation:
Walai Rukhavej Botanical Research Institute (WRBRI), Mahasarakham University, Mahasarakham 44150, Thailand
P. SITHITHAWORN*
Affiliation:
Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand Liver Fluke and Cholangiocarcinoma Research Center (LFCRC), Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand
N. B. CHILTON
Affiliation:
Department of Biology, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5E2, Canada
T. N. PETNEY
Affiliation:
Institute of Zoology 1: Ecology and Parasitology, University of Karlsruhe, Kornblumen Strasse 13, Karlsruhe, Germany
S. KLINBUNGA
Affiliation:
National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), 113 Paholyothin Road, Klong 1, Klong Luang, Pathumthani 12120, Thailand
R. SATRAWAHA
Affiliation:
Walai Rukhavej Botanical Research Institute (WRBRI), Mahasarakham University, Mahasarakham 44150, Thailand
J. P. WEBSTER
Affiliation:
Department of Infectious Disease Epidemiology, Imperial College Faculty of Medicine, Norfolk Place, London W2 1PG, UK
R. H. ANDREWS
Affiliation:
School of Pharmacy and Medical Sciences, University of South Australia, GPO Box 2471, Adelaide, SA 5001, Australia
*
*Corresponding author: Department of Parasitology and Liver Fluke and Cholangiocarcinoma Research Center, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand. Tel: +66 43348387. Fax: +66 43202475. E-mail: paib_sit@kku.ac.th
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Summary

The population genetics of 317 individual Opisthorchis viverrini from Khon Kaen Province Thailand, from 4 different years and 4 cyprinid fish species was examined using multilocus enzyme electrophoresis of enolase (Enol), phosphoglucomutase (Pgm) and triose phosphate isomerase (Tpi). Allele and genotype frequencies for Enol and Pgm were consistent irrespective of year or host species. No heterozygote deficiency was detected for Enol. Significant heterozygote deficiencies were detected in 3 of 4 years for Pgm. For Tpi, allele frequencies of the most common allele and genotype frequency varied between years and among individuals from different host species. Heterozygote deficiencies for Tpi were detected in 2 years. No significant heterozygous deficiencies were detected among O. virerrini from different fish species in 2005, except at Pgm and Tpi from Puntioplites protozsron. There was no statistical significance in pairwise FST values between O. viverrini from Cyclocheilichthys armatus in different years or different host species in 2005. Significant departures from Hardy-Weinberg expectations and a high rate of gene flow in a population of O. viverrini are discussed in terms of self- and cross-fertilisation, natural selection, non-random mating, the Wahlund effect, presence of null alleles, intensity of infection, biology and ecology of their intermediate cyprinid hosts.

Keywords

Opisthorchis viverrinitemporal variationpopulation geneticsallozyme markers
Type
Research Article
Information
Parasitology , Volume 136 , Issue 9 , August 2009 , pp. 1057 - 1063
Copyright
Copyright © Cambridge University Press 2009

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