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In vitro anthelmintic activity of an R-carvone nanoemulsions towards multiresistant Haemonchus contortus

Published online by Cambridge University Press:  02 September 2022

Antônia Aniellen Raianne Moises Aguiar ,
José Vilemar de Araújo Filho ,
Henety Nascimento Pinheiro ,
Matheus da Silva Campelo ,
Wesley Lyeverton Correia Ribeiro ,
Ana Carolina Fonseca Lindoso Melo ,
Letícia Oliveira da Rocha ,
Maria Elenir Nobre Pinho Ribeiro ,
Nágila Maria Pontes Silva Ricardo  and
Flávia Oliveira Monteiro da Silva Abreu
...Show all authors
Antônia Aniellen Raianne Moises Aguiar
Affiliation:
Laboratório de Doenças Parasitárias, Programa de Pós-Graduação em Ciências Veterinárias, Universidade Estadual do Ceará, Fortaleza, Brazil
José Vilemar de Araújo Filho
Affiliation:
Laboratório de Doenças Parasitárias, Programa de Pós-Graduação em Ciências Veterinárias, Universidade Estadual do Ceará, Fortaleza, Brazil
Henety Nascimento Pinheiro
Affiliation:
Laboratório de Química Analítica e Ambiental, Programa de Pós-Graduação em Ciências Naturais, Universidade Estadual do Ceará, Fortaleza, Brazil
Matheus da Silva Campelo
Affiliation:
Laboratório de Polímeros e Inovação de Materiais, Departamento de Química Orgânica e Inorgânica, Universidade Federal do Ceará, Fortaleza, Brazil
Wesley Lyeverton Correia Ribeiro
Affiliation:
Departamento de Fisiologia e Farmacologia, Faculdade de Medicina, Universidade Federal do Ceará, Fortaleza, Brazil
Ana Carolina Fonseca Lindoso Melo
Affiliation:
Departamento de Patologia e Medicina Legal, Faculdade de Medicina, Universidade Federal do Ceará, Fortaleza, Brazil
Letícia Oliveira da Rocha
Affiliation:
Laboratório de Biologia Celular e Tecidual, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense, Rio de Janeiro, Brazil Laboratório de Ecotoxicologia, Centro de Ciências Exatas, Naturais e da Saúde, Universidade Federal do Espírito Santo, Vitória, Brazil
Maria Elenir Nobre Pinho Ribeiro
Affiliation:
Laboratório de Polímeros e Inovação de Materiais, Departamento de Química Orgânica e Inorgânica, Universidade Federal do Ceará, Fortaleza, Brazil
Nágila Maria Pontes Silva Ricardo
Affiliation:
Laboratório de Polímeros e Inovação de Materiais, Departamento de Química Orgânica e Inorgânica, Universidade Federal do Ceará, Fortaleza, Brazil
Flávia Oliveira Monteiro da Silva Abreu
Affiliation:
Laboratório de Química Analítica e Ambiental, Programa de Pós-Graduação em Ciências Naturais, Universidade Estadual do Ceará, Fortaleza, Brazil
Lorena Mayana Beserra de Oliveira
Affiliation:
Laboratório de Doenças Parasitárias, Programa de Pós-Graduação em Ciências Veterinárias, Universidade Estadual do Ceará, Fortaleza, Brazil
Weibson Paz Pinheiro André
Affiliation:
Laboratório de Doenças Parasitárias, Programa de Pós-Graduação em Ciências Veterinárias, Universidade Estadual do Ceará, Fortaleza, Brazil
Claudia Maria Leal Bevilaqua*
Affiliation:
Laboratório de Doenças Parasitárias, Programa de Pós-Graduação em Ciências Veterinárias, Universidade Estadual do Ceará, Fortaleza, Brazil
*
Author for correspondence: Claudia Maria Leal Bevilaqua, E-mail: bevilaqua.uece@gmail.com
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Abstract

This work aimed to evaluate the in vitro anthelmintic effect of carvone nanoemulsions on Haemonchus contortus. Three R-carvone nanoemulsions were prepared: uncoated R-carvone nanoemulsions homogenized in a sonicator (UNAlg-son) and homogenized in an ultrahomogenizer (UNAlg-ultra) and sodium alginate-coated R-carvone (CNAlg-ultra). The physicochemical characterizations of the nanoemulsions were carried out. The anthelmintic activity was evaluated using egg hatch test (EHT), larval development test (LDT) and adult worm motility test (AWMT). Changes in cuticle induced in adult H. contortus were evaluated by scanning electron microscopy (SEM). The results were subjected to analysis of variance and compared using the Tukey test (P < 0.05). The effective concentration to inhibit 50% (EC50) of egg hatching and larval development was calculated. The particle sizes were 281.1 nm (UNAlg-son), 152.7 nm (UNAlg-ultra) and 557.8 nm (CNAlg-ultra), and the zeta potentials were −15 mV (UNAlg-son), −10.8 mV (UNAlg-ultra) and −24.2 mV (CNAlg-ultra). The encapsulation efficiency was 99.84 ± 0.01%. SEM of the nanoemulsions showed an increase in size. In EHT, the EC50 values of UNAlg-son, UNAlg-ultra and CNAlg-ultra were 0.19, 0.02 and 0.17 mg mL−1, respectively. In LDT, they were 0.29, 0.31 and 0.95 mg mL−1 for UNAlg-son, UNAlg-ultra and CNAlg-ultra, respectively. The adult motility inhibition was 100% after 12 h of exposure to UNAlg-ultra and CNAlg-ultra, while for UNAlg-son, it was 79.16%. SEM showed changes in the buccal capsule and cuticular damage. It was concluded that R-carvone nanoemulsions showed antiparasitic action demonstrating promise for the control of infections caused by gastrointestinal nematodes in small ruminants.

Keywords

Carvonenanoemulsionnematodesphytotherapysodium alginate
Type
Research Article
Information
Parasitology , Volume 149 , Issue 12 , October 2022 , pp. 1631 - 1641
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Copyright
Copyright © The Author(s), 2022. Published by Cambridge University Press

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