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In vitro inhibition of Plasmodium falciparum merozoite invasion by human plasma fibronectin

Published online by Cambridge University Press:  06 April 2009

M. A. Ouaissi ,
E. Dutoit ,
A. Vernes ,
S. Guerret-Stocker  and
A. Capron
M. A. Ouaissi
Affiliation:
Centre d'immunologie et de Biologie Parasitaire, INSERM U 167 - CNRS 624, 15 rue Camille Guérin, 59019 Lille Cédex, France
E. Dutoit
Affiliation:
Unité 42 INSERM, 369 rue Jules Guesde, Flers, 59650 Villeneuve d'ascq, France
A. Vernes
Affiliation:
Unité 42 INSERM, 369 rue Jules Guesde, Flers, 59650 Villeneuve d'ascq, France
S. Guerret-Stocker
Affiliation:
Laboratoire de Pathologie Cellulaire du Foie, CNRS ERA 819, Institut Pasteur, 77 rue Pasteur, 69365 Lyon, France
A. Capron
Affiliation:
Centre d'immunologie et de Biologie Parasitaire, INSERM U 167 - CNRS 624, 15 rue Camille Guérin, 59019 Lille Cédex, France
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Extract

Human fibronectin isolated from citrated blood was tested for its ability to bind to Plasmodium falciparum by an indirect immunofluorescent assay using rabbit antiserum to human fibronectin. A positive reaction was observed on merozoites inside schizont-infected erythrocytes. The binding was not observed on non-parasitized red blood cells. The effect of human fibronectin on P.falciparum growth was further studied using an in vitro inhibition assay; 50% inhibition of parasite multiplication was obtained with approximately 100 μg/ml of human fibronectin. Slight inhibition was observed below 10 μg/ml. The significance of this finding is discussed.

Type
Research Article
Information
Parasitology , Volume 90 , Issue 2 , April 1985 , pp. 227 - 236
Copyright
Copyright © Cambridge University Press 1985

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