Hostname: page-component-cd9895bd7-dk4vv Total loading time: 0 Render date: 2024-12-26T06:48:58.777Z Has data issue: false hasContentIssue false
  • English
  • Français

Induction of immunity in sheep to Fasciola hepatica with mimotopes of cathepsin L selected from a phage display library

Published online by Cambridge University Press:  24 September 2008

A. VILLA-MANCERA ,
H. QUIROZ-ROMERO ,
D. CORREA ,
F. IBARRA ,
M. REYES-PÉREZ ,
H. REYES-VIVAS ,
G. LÓPEZ-VELÁZQUEZ ,
K. GAZARIAN ,
T. GAZARIAN  and
R. A. ALONSO
A. VILLA-MANCERA
Affiliation:
Departamento de Parasitología, Facultad de Medicina Veterinaria y Zootecnia, UNAM, México, D.F. 04510
H. QUIROZ-ROMERO*
Affiliation:
Departamento de Parasitología, Facultad de Medicina Veterinaria y Zootecnia, UNAM, México, D.F. 04510
D. CORREA
Affiliation:
Laboratorio de Inmunología Experimental, Instituto Nacional de Pediatría, México, D.F. 04530
F. IBARRA
Affiliation:
Departamento de Parasitología, Facultad de Medicina Veterinaria y Zootecnia, UNAM, México, D.F. 04510
M. REYES-PÉREZ
Affiliation:
Departamento de Parasitología, Facultad de Medicina Veterinaria y Zootecnia, UNAM, México, D.F. 04510
H. REYES-VIVAS
Affiliation:
Laboratorio de Bioquímica Genética, Instituto Nacional de Pediatría, México, D.F. 04530
G. LÓPEZ-VELÁZQUEZ
Affiliation:
Laboratorio de Bioquímica Genética, Instituto Nacional de Pediatría, México, D.F. 04530
K. GAZARIAN
Affiliation:
Departamento de Biología Molecular y Biotecnología, Instituto de Investigaciones Biomédicas, UNAM, México, D.F. 04510
T. GAZARIAN
Affiliation:
Departamento de Biología Molecular y Biotecnología, Instituto de Investigaciones Biomédicas, UNAM, México, D.F. 04510
R. A. ALONSO
Affiliation:
Departamento de Genética y Bioestadística, Facultad de Medicina Veterinaria y Zootecnia, UNAM, México, D.F. 04510
*
*Corresponding author: Departamento de Parasitología, Facultad de Medicina Veterinaria y Zootecnia, UNAM, México, D.F. 04510. Tel: +52 56225898. Fax: +52 56225898. E-mail: hquiroz@servidor.unam.mx
Get access Rights & Permissions [Opens in a new window]

Summary

An M13 phage random 12-mers peptide library was used to screen cathepsin L mimotopes of Fasciola hepatica and to evaluate their immunogenicity in sheep. Seven clones showed positive reactivity to a rabbit anti-cathepsin L1/L2 antiserum in ELISA, and their amino acid sequences deduced by DNA sequencing were tentatively mapped on the protein. Twenty sheep were randomly allocated into 4 groups of 5 animals each, for immunization with 1×1014 phage particles of clones 1, 20, a mixture of 7 clones and PBS, without adjuvant at the beginning, and 4 weeks later. All groups were challenged with 300 metacercariae at week 6 and slaughtered 16 weeks later. The mean worm burdens after challenge were reduced by 47·61% and 33·91% in sheep vaccinated with clones 1 and 20, respectively; no effect was observed in animals inoculated with the clone mixture. Also, a significant reduction in worm size and burden was observed for those sheep immunized with clone 1. Animals receiving clone 20, showed a significant reduction in egg output. Immunization induced a reduction of egg viability ranging from 58·92 to 82·11%. Furthermore, vaccinated animals produced clone-specific antibodies which were boosted after challenge with metacercariae of F. hepatica.

Keywords

Fasciola hepaticacathepsin Lphage displaymimotopesvaccine
Type
Original Articles
Information
Parasitology , Volume 135 , Issue 12 , October 2008 , pp. 1437 - 1445
Copyright
Copyright © 2008 Cambridge University Press

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Berasaín, P., Goni, F., McGonigle, S., Dowd, A., Dalton, J. P., Frangione, B. and Carmona, C. (1997). Proteinases secreted by Fasciola hepatica degrade extracellular matrix and basement membrane components. Journal of Parasitology 83, 1–5.CrossRefGoogle ScholarPubMed
Bradford, M. M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72, 248–254.CrossRefGoogle ScholarPubMed
Carmona, C., Dowd, A. J., Smith, A. M. and Dalton, J. P. (1993). Cathepsin L proteinase secreted by Fasciola hepatica in vitro prevents antibody-mediated eosinophil attachment to newly excysted juveniles. Molecular and Biochemical Parasitology 62, 9–17.CrossRefGoogle ScholarPubMed
Dalton, J. P. and Heffernan, M. (1989). Thiol proteases released in vitro by Fasciola hepatica. Molecular and Biochemical Parasitology 35, 161–166.CrossRefGoogle ScholarPubMed
Dalton, J. P., McGonigle, S., Rolph, T. P. and Andrews, S. J. (1996). Induction of protective immunity in cattle against infection with Fasciola hepatica by vaccination with cathepsin L proteinases and with hemoglobin. Infection and Immunity 64, 5066–5074.CrossRefGoogle ScholarPubMed
Dalton, J. P., Neill, S. O., Stack, C., Collins, P., Walshe, A., Sekiya, M., Doyle, S., Mulcahy, G., Hoyle, D., Khaznadji, E., Moiré, N., Brennan, G., Mousley, A., Kreshchenko, N., Maule, A. G. and Donnelly, S. M. (2003). Fasciola hepatica cathepsin L-like proteases: biology, function, and potential in the development of first generation liver fluke vaccines. International Journal for Parasitology 33, 1173–1181.CrossRefGoogle ScholarPubMed
Demangel, C., Lafaye, P. and Mazie, J. C. (1996). Reproducing the immune response against the Plasmodium vivax merozoite surface protein 1 with mimotopes selected from a phage-displayed peptide library. Molecular Immunology 33, 909–916.CrossRefGoogle ScholarPubMed
Gaasenbeek, C. P., Moll, L., Cornelissen, J. B., Vellema, P. and Borgsteede, F. H. (2001). An experimental study on triclabendazole resistance of Fasciola hepatica in sheep. Veterinary Parasitology 95, 37–43.CrossRefGoogle Scholar
Harmsen, M. M., Cornelissen, J. B., Buijs, H. E., Boersma, W. J., Jeurissen, S. H. and van Milligen, F. J. (2004). Identification of a novel Fasciola hepatica cathepsin L protease containing protective epitopes within the propeptide. International Journal for Parasitology 34, 675–682.CrossRefGoogle ScholarPubMed
Hillyer, G. V. (2005). Fasciola antigens as vaccines against fascioliasis and schistosomiasis. Journal of Helminthology 79, 241–247.CrossRefGoogle ScholarPubMed
Huang, J., Gutteridge, A., Honda, W. and Kanehisa, M. (2006). MIMOX: a web tool for phage display based epitope mapping. BMC Bioinformatics 12, 451.CrossRefGoogle Scholar
Mas-Coma, S., Burgues, M. D. and Esteban, J. G. (1999). Human fasciolosis. In Fasciolosis (ed. Dalton, J. P.), pp. 411–434. CAB International, Wallingford, Oxon, UK.Google Scholar
Mas-Coma, S. (2005). Epidemiology of fascioliasis in human endemic areas. Journal of Helminthology 79, 207–216.CrossRefGoogle ScholarPubMed
McManus, D. P. and Dalton, J. P. (2006). Vaccines against the zoonotic trematodes Schistosoma japonicum, Fasciola hepatica and Fasciola gigantica. Parasitology 133, S43–S61.CrossRefGoogle ScholarPubMed
Mitchell, G. B., Maris, L. and Bonniwell, M. A. (1998). Triclabendazole-resistant liver fluke in Scottish sheep. The Veterinary Record 143, 399.Google ScholarPubMed
Moll, L., Gaasenbeek, C. P., Vellema, P. and Borgsteede, F. H. (2000). Resistance of Fasciola hepatica against triclabendazole in cattle and sheep in The Netherlands. Veterinary Parasitology 91, 153–158.CrossRefGoogle ScholarPubMed
Narum, D. L., Ogun, S. A., Batchelor, A. H. and Holder, A. A. (2006). Passive immunization with a multicomponent vaccine against conserved domains of apical membrane antigen 1 and 235-kilodalton rhoptry proteins protects mice against Plasmodium yoelii blood-stage challenge infection. Infection and Immunity 74, 5529–5536.CrossRefGoogle ScholarPubMed
Overend, D. J. and Bowen, F. L. (1995). Resistance of Fasciola hepatica to triclabendazole. Australian Veterinary Journal 72, 275–276.CrossRefGoogle ScholarPubMed
Piacenza, L., Acosta, D., Basmadjian, I., Dalton, J. P. and Carmona, C. (1999). Vaccination with cathepsin L proteinases and with leucine aminopeptidase induces high levels of protection against fascioliasis in sheep. Infection and Immunity 67, 1954–1961.CrossRefGoogle ScholarPubMed
Sexton, J. L., Milner, A. R., Panaccio, M., Waddington, J., Wijffels, G., Chandler, D., Thompson, C., Wilson, L., Spithill, T. W., Mitchell, G. F. and Campbell, N. J. (1990). Glutathione S-transferase. Novel vaccine against Fasciola hepatica infection in sheep. Journal of Immunology 145, 3905–3910.CrossRefGoogle ScholarPubMed
Shevchenko, A., Wilm, M., Vorm, O. and Mann, M. (1996). Mass spectrometric sequencing of proteins silver-stained polyacrylamide gels. Analytical Chemistry 68, 850–858.CrossRefGoogle ScholarPubMed
Smith, A. M., Carmona, C., Dowd, A. J., McGonigle, S., Acosta, D. and Dalton, J. P. (1994). Neutralization of the activity of a Fasciola hepatica cathepsin L proteinase by anti-cathepsin L antibodies. Parasite Immunology 16, 325–328.CrossRefGoogle ScholarPubMed
Smith, A. M., Dowd, A. J., Heffernan, M., Robertson, C. D. and Dalton, J. P. (1993). Fasciola hepatica: a secreted cathepsin L-like proteinase cleaves host immunoglobulin. International Journal for Parasitology 23, 977–983.CrossRefGoogle ScholarPubMed
Smith, G. P. and Petrenko, V. A. (1997). Phage display. Chemical Reviews 97, 391–410.CrossRefGoogle ScholarPubMed
Tang, L. F., Yi, X. Y., Zeng, X. F., Wang, L. Q. and Zhang, S. K. (2004). Schistosoma japonicum: isolation and identification of peptides mimicking ferritin epitopes from phage display library. Acta Biochimica et Biophysica Sinica 36, 206–210.CrossRefGoogle ScholarPubMed
Torgerson, P. R. and Claxton, J. R. (1999). Epidemiology and control. In Fasciolosis (ed. Dalton, J. P.), pp. 113–149. CAB International, Wallingford, Oxon, UK.Google Scholar
Wang, M., Yi, X. Y., Li, X. P., Zhou, D. M., Larry, M. and Zeng, X. F. (2005). Phage displaying peptides mimic schistosoma antigenic epitopes selected by rat natural antibodies and protective immunity induced by their immunization in mice. World Journal of Gastroenterology 11, 2960–2966.CrossRefGoogle ScholarPubMed
Wijffels, G. L., Salvatore, L., Dosen, M., Waddington, J., Wilson, L., Thompson, C., Campbell, N., Sexton, J., Wicker, J., Bowen, F., Friedel, T. and Spithill, T. W. (1994). Vaccination of sheep with purified cysteine proteinases of Fasciola hepatica. Experimental Parasitology 78, 132–148.CrossRefGoogle ScholarPubMed
Wu, H. W., Hu, X. M., Wang, Y., Kurtis, J. D., Zeng, F. J., McGarvey, S. T., Wu, G. L., Zhang, Z. S. and Hua, Z. C. (2006). Protective immunity induced by phage displayed mitochondrial related peptides of Schistosoma japonicum. Acta Tropica 99, 200–207.CrossRefGoogle ScholarPubMed
Yamasaki, H., Aoki, T. and Oya, H. (1989). A cysteine proteinase from the liver fluke Fasciola spp.: purification, characterization, localization and application to immunodiagnosis. Japanese Journal of Parasitology 38, 373–384.Google Scholar