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Inhibition of gametogenesis by the cestode Ligula intestinalis in roach (Rutilus rutilus) is attenuated under laboratory conditions

Published online by Cambridge University Press:  22 November 2010

ACHIM TRUBIROHA ,
HANA KROUPOVA ,
SABRINA N. FRANK ,
BERND SURES  and
WERNER KLOAS
ACHIM TRUBIROHA*
Affiliation:
Department of Ecophysiology and Aquaculture, Leibniz-Institute of Freshwater Ecology and Inland Fisheries, Mueggelseedamm 310, D-12587 Berlin, Germany
HANA KROUPOVA
Affiliation:
Department of Ecophysiology and Aquaculture, Leibniz-Institute of Freshwater Ecology and Inland Fisheries, Mueggelseedamm 310, D-12587 Berlin, Germany Research Institute of Fish Culture and Hydrobiology, Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, Zatisi 728/II, 38925 Vodnany, Czech Republic
SABRINA N. FRANK
Affiliation:
Department of Ecophysiology and Aquaculture, Leibniz-Institute of Freshwater Ecology and Inland Fisheries, Mueggelseedamm 310, D-12587 Berlin, Germany Department of Applied Zoology/Hydrobiology, University of Duisburg/Essen, Universitaetsstrasse 5, D-45141 Essen, Germany
BERND SURES
Affiliation:
Department of Applied Zoology/Hydrobiology, University of Duisburg/Essen, Universitaetsstrasse 5, D-45141 Essen, Germany
WERNER KLOAS
Affiliation:
Department of Ecophysiology and Aquaculture, Leibniz-Institute of Freshwater Ecology and Inland Fisheries, Mueggelseedamm 310, D-12587 Berlin, Germany Department of Endocrinology, Humboldt University Berlin, Invalidenstrasse 42, D-10099 Berlin, Germany
*
*Corresponding author: Department of Ecophysiology and Aquaculture, Leibniz-Institute of Freshwater Ecology and Inland Fisheries, Mueggelseedamm 301, D-12587 Berlin, Germany. Tel: +49 30 64181 614. Fax: +49 30 64181 799. E-mail: trubiroha@igb-berlin.de
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Summary

Reproductive parameters of Ligula intestinalis-infected roach (Rutilus rutilus) which were held under long-tem laboratory conditions with unlimited food supply were investigated. Although uninfected and infected roach showed no difference in condition factor and both groups deposited perivisceral fat, the gonadosomatic-index was significantly lower in infected female and male roach. Quantitative histological analysis revealed that gonad development was retarded upon parasitization in both genders. In contrast to the phenotype described in the field, infected females were able to recruit follicles into secondary growth, but a high percentage of secondary growth follicles underwent atresia. In both genders, the histological data corresponded well with reduced expression of pituitary gonadotropins and lowered plasma concentrations of sex steroids, as revealed by real-time RT-PCR and ELISA, respectively. Furthermore, a reduction of vitellogenin mRNA and modulated expression of sex steroid receptors in the liver was demonstrated. Like in the field, there was a significant adverse impact of L. intestinalis on host reproductive physiology which could not be related to parasite burden. Our results show, for the first time, that maintenance under laboratory conditions can not abolish the deleterious effect of L. intestinalis on gametogenesis in roach, and indicate a specific inhibition of host reproduction by endocrine disruption.

Keywords

parasitetapewormhost fecundityparasitic castrationendocrine disruptiongonadotropinsex steroidsoestrogenandrogenvitellogenin
Type
Research Article
Information
Parasitology , Volume 138 , Issue 5 , April 2011 , pp. 648 - 659
Copyright
Copyright © Cambridge University Press 2010

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