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Interactions of Plasmodium juxtanucleare and chicken anaemia virus: establishing a model

Published online by Cambridge University Press:  19 August 2013

PATRICIA SILVEIRA ,
SANDRA Y. G. MARIN ,
PATRICIA A. MOREIRA ,
BÁRBARA B. TOCANTINS ,
GUSTAVO LACORTE ,
TATIANE A. PAIXÃO ,
NELSON R. S. MARTINS  and
ÈRIKA M. BRAGA
PATRICIA SILVEIRA
Affiliation:
Departamento de Parasitologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG 31270-901, Brazil
SANDRA Y. G. MARIN
Affiliation:
Setor de Doenças das Aves, Departamento de Medicina Veterinária Preventiva, Escola de Veterinária, Belo Horizonte, MG 31270-901, Brazil
PATRICIA A. MOREIRA
Affiliation:
Departamento de Parasitologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG 31270-901, Brazil
BÁRBARA B. TOCANTINS
Affiliation:
Faculdade de Agronomia e Medicina Veterinária, Universidade de Brasília, Campus Universitário Darcy Ribeiro, Brasília, DF 70910-900, Brazil
GUSTAVO LACORTE
Affiliation:
Departamento de Ciências Agrárias, Instituto Federal de Minas Gerais, Escola Agrotécnica Federal de Bambuí, MG 38900-000, Brazil
TATIANE A. PAIXÃO
Affiliation:
Departamento de Patologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG 31270-901, Brazil
NELSON R. S. MARTINS
Affiliation:
Setor de Doenças das Aves, Departamento de Medicina Veterinária Preventiva, Escola de Veterinária, Belo Horizonte, MG 31270-901, Brazil
ÈRIKA M. BRAGA*
Affiliation:
Departamento de Parasitologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG 31270-901, Brazil
*
*Corresponding author: Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627. Instituto de Ciências Biológicas, Departamento de Parasitologia, Belo Horizonte, MG 31270-910, Brazil. E-mail: embraga@icb.ufmg.br
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Summary

The pathogens Plasmodium juxtanucleare and chicken anaemia virus (CAV) are easily transmitted and potentially harmful to chickens. In this study, we established an experimental model to investigate the effects of avian malaria caused by P. juxtanucleare in white leghorn specific-pathogen-free (SPF) chicks previously immunosuppressed with CAV. Parasitaemia, haematological variables and clinical and pathological parameters were determined in four different experimental groups: chicks coinfected by CAV and P. juxtanucleare strain (Coinfected group), chicks exclusively infected by CAV (CAV group) or P. juxtanucleare (Malaria group) and uninfected chicks (Control group). Our data demonstrated that P. juxtanucleare parasitaemia was significantly higher in the Coinfected group. Furthermore, haematological parameters, including the RBC, haematocrit and haemoglobin concentration were significantly reduced in coinfected chicks. In agreement with the changes observed in haematological features, the mortality among coinfected chicks was higher compared with animals with single infections. Clinical analysis indicated moderate changes related to different organs size (bursa of Fabricius, heart and liver) in coinfected birds. The experimental coinfection of SPF chickens with P. juxtanucleare and CAV may represent a research tool for the study of avian malaria after CAV immunosuppression, enabling measurement of the impacts caused by different pathogens during malarial infection.

Keywords

Plasmodium juxtanuclearechicken anaemia virusanaemia
Type
Research Article
Information
Parasitology , Volume 140 , Issue 14 , December 2013 , pp. 1777 - 1788
Copyright
Copyright © Cambridge University Press 2013 

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