Hostname: page-component-78c5997874-t5tsf Total loading time: 0 Render date: 2024-11-10T12:06:52.621Z Has data issue: false hasContentIssue false
  • English
  • Français

Interspecific differences in the nematode surface coat between Meloidogyne incognita and M. arenaria related to the adhesion of the bacterium Pasteuria penetrans

Published online by Cambridge University Press:  06 April 2009

K. G. Davies  and
C. Danks
K. G. Davies
Affiliation:
AFRC Institute of Arable Crops Research, Rothamsted Experimental Station, Harpenden, Hertfordshire AL5 2JQ
C. Danks
Affiliation:
AFRC Institute of Arable Crops Research, Rothamsted Experimental Station, Harpenden, Hertfordshire AL5 2JQ
Get access Rights & Permissions [Opens in a new window]

Summary

Spores of the bacterium Pasteuria penetrans adhered to second-stage juveniles of both Meloidogyne incognita and M. arenaria, but in standard attachment assays far fewer adhered to the latter species. Similarly, a polyclonal antibody was shown not to recognize the surface coat of M. arenaria but did recognize the surface coat of M. incognita. Although the incubation of whole, intact 2nd-stage juveniles of M. incognita in a series of detergents and protein-denaturing agents at room temperature did not reduce the number of spores adhering after exposure to the bacterium, incubation in buffer (PBS) alone at 100 °C for 2 min did. Immunoblotting of cuticle extracts onto nitrocellulose and probing the blots with antibody showed that the antibody recognized a large protein of Mr 80 kDa and a series of smaller proteins of approximately 43 kDa on M. incognita which were not recognized in extracts from M. arenaria. As incubation of the 2nd-stage juveniles in antibody prohibited spore attachment it is suggested that these proteins may be involved in spore adhesion. Large differences exist between M. incognita and M. arenaria in the amounts of surface-associated proteins on the 2nd-stage juvenile cuticle.

Keywords

root-knot nematodeglycocalyxcuticlebiological control
Type
Research Article
Information
Parasitology , Volume 105 , Issue 3 , December 1992 , pp. 475 - 480
Copyright
Copyright © Cambridge University Press 1992

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Bird, A. F. (1985). The nature of the adhesion of Corynebacterium rathayi to the cuticle of the infective larva of Anguina agrostis. International Journal for Parasitology 15, 301–8.CrossRefGoogle Scholar
Bird, A. F., Bonig, I. & Bacic, A. (1989). Factors affecting the adhesion of micro-organisms to the surfaces of plant-parasitic nematodes. Parasitology 15, 301–8.Google Scholar
Bird, A. F. & Zuckerman, B. M. (1989). Studies on the surface coat (glycocalyx) of the dauer larva of Anguina agrostis. International Journal for Parasitology 19, 235–40.CrossRefGoogle ScholarPubMed
Davies, K. G. & Danks, C. (1992). Carbohydrate/protein interactions between the cuticle of infective juveniles of Meloidogyne incognita and spores of the obligate hyperparasite Pasteuria penetrans. Nematologica (in the Press).Google Scholar
Davies, K. G., Kerry, B. R. & Flynn, C. A. (1988). Observations on the pathogenicity of Pasteuria penetrans a parasite of root-knot nematodes. Annals of Applied Biology 112, 491–501.CrossRefGoogle Scholar
Davies, K. G. & Lander, E. B. (1992). Immunological differentiation of root-knot nematodes (Meloidogyne spp.) using monoclonal and polyclonal antibodies. Nematologica (in the Press).Google Scholar
Davies, K. G., Leij, DE F., A. A., M. & Kerry, B. R. (1991). Microbial agents for the biological control of plant-parasitic nematodes in tropical agriculture. Tropical Pest Management 37, 303–20.CrossRefGoogle Scholar
Davies, K. G., Robinson, M. P. & Laird, V. (1992). Proteins involved in the attachment of a hyperparasite, Pasteuria penetrans, to its plant-parasitic nematode host, Meloidogyne incognita. Journal of Invertebrate Pathology 59, 18–23.CrossRefGoogle Scholar
Eisenback, J. D. & Triantaphyllou, H. H. (1991). Root-knot nematodes: Meloidogyne species and races. In Manual of Agricultural Nematology (ed. Nickle, W. R.), pp. 191–274. New York: Marcel Dekker.Google Scholar
Finne, J. & Krusius, T. (1982). Preparation and fractionation of glycolipids. Methods in Enzymology 83, 269–77.CrossRefGoogle Scholar
Hooper, D. J. (1986). Extraction of free-living stages from soil. In Laboratory Methods for Working with Plant and Soil Nematodes (ed. Southey, J. F.). MAFF, HMSO Ref Book 402.Google Scholar
Johnson, G. D. & NOGUEIRA-Araujo, G. M. DE (1981). A simple method of reducing the fading immunofluorescence during microscopy. Journal of Immunological Methods 43, 349–50.CrossRefGoogle ScholarPubMed
Laemmli, U. K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227, 680–5.CrossRefGoogle ScholarPubMed
Mcclure, M. A. & Spiegel, Y. (1991). Role of the nematode surface coat in the adhesion of Clavibacter sp. to Anguina funestra and Anguina tritici. Parasitology 103, 421–7.CrossRefGoogle Scholar
Persidis, A., Lay, J. G., Manousis, T., Bishop, A. H. & Ellar, D. J. (1991). Characterisation of potential adhesions of the bacterium Pasteuria penetrans, and of putitive receptors on the cuticle of Meloidogyne incognita, a nematode host. Journal of Cell Science 100, 613–22.Google Scholar
Politz, S. M. & Philipp, M. (1992). Caenorhabditis elegans as a model for parasitic nematodes: a focus on the cuticle. Parasitology Today 8, 6–12.CrossRefGoogle Scholar
Proudfoot, L., Kusel, J. R., Smith, H. V. & Kennedy, M. W. (1991). Biophysical properties of the nematode surface. In Parasitic Nematodes–Antigens, Membranes and Genes (ed. Kennedy, M. W.), pp. 1–26. London: Taylor & Francis.Google Scholar
Reddigari, S. R., Jansma, P. L., Premachandran, D. & Hussey, R. S. (1986). Cuticular collagenous proteins of second-stage juveniles and adult females of Meloidogyne incognita: isolation and partial characterisation. Journal of Nematology 18, 294–302.Google Scholar
Robinson, M. P., Delgado, J. & Parkhouse, R. M. E. (1989). Characterisation of stage-specific cuticular proteins of Meloidogyne incognita by radio-iodination. Physiological and Molecular Plant Pathology 35, 135–40.CrossRefGoogle Scholar
Stirling, G. R. (1991). Biological Control of Plant-Parasitic Nematodes: Progress, Problems and Prospects. Wallingford: CABI.Google Scholar
Stirling, G. R. & Wachtel, M. F. (1980). Mass production of Bacillus penetrans for the biological control of root-knot nematodes. Nematologica 26, 308–12.CrossRefGoogle Scholar
Thomason, I. J. (1987). Challenges facing nematology: environmental risks with nematicides and the need for new approaches. In Vistas on Nematology (ed. Veech, J. A. & Dickson, D. W.), pp. 469–476. Hyattsville: Society of Nematologists.Google Scholar
Wright, K. A. (1987). The nematode's cuticle – its surface and the epidermis: function, homology analogy – a current consensus. Journal of Parasitology 73, 1077–83.CrossRefGoogle ScholarPubMed