Hostname: page-component-cd9895bd7-mkpzs Total loading time: 0 Render date: 2024-12-27T11:14:59.568Z Has data issue: false hasContentIssue false

Invasion of erythrocytes by Plasmodium falciparum in vitro

Published online by Cambridge University Press:  06 April 2009

P. I. Trigg
Affiliation:
Division of Parasitology, National Institute for Medical Research, Mill Hill, London NW7 1AA

Extract

The erythrocytic stages of Plasmodium falciparum from Aotus trivirgatus were grown in Mill Hill medium. Significant invasion of erythrocytes was obtained by a subculture technique. This invasion, by parasites grown in Aotus erythrocytes, was greater when human erythrocytes were added to the cultures than when parasites were allowed to invade Aotus erythrocytes. This difference appeared to be a function of the properties of the erythrocyte and was not serum dependent.

Type
Research Article
Copyright
Copyright © Cambridge University Press 1975

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Butcher, G. A., Mitchell, G. H. & Cohen, S. (1973). Mechanism of host specificity in malarial infection. Nature, London 244, 40–2.CrossRefGoogle ScholarPubMed
Diggs, C. L., Pavanand, K., Permpanich, B., Numsuwankijkul, V., Haup, R. & Chuanak, N. (1971). Penetration of human fetal erythrocytes by Plasmodium falciparum in vitro. Journal of Parasitology 57, 187–8.CrossRefGoogle ScholarPubMed
Geiman, Q. M., Siddiqui, W. A. & Schnell, J. V. (1966). Plasma replacement for in vitro culture of Plasmodium knowlesi. Science 153, 1129–30.CrossRefGoogle ScholarPubMed
Phillips, R. S., Trigg, P. I., Scott-Finnigan, T. J. & Bartholomew, R. K. (1972). Culture of Plasmodium falciparum in vitro, a subculture technique used for demonstrating antiplasmodial activity in serum from some Gambians, resident in an endemic malarious area. Parasitology 65, 525–35.CrossRefGoogle Scholar
Siddiqui, W. A., Geiman, Q. M., Schnell, J. V. (1970). Use of a commercially available culture medium to test the susceptibility of human anti-malarial drugs. Journal of Parasitology 56, 188–9.CrossRefGoogle Scholar
Siddiqui, W. A., Schnell, J. V. & Geiman, Q. M. (1970). In vitro cultivation of Plasmodium falciparum. American Journal of Tropical Medicine and Hygiene 19, 586–91.CrossRefGoogle ScholarPubMed
Siddiqui, W. A. & Schnell, J. V. (1973). Use of various buffers for in vitro cultivation of malarial parasites. Journal of Parasitology 59, 516–9.CrossRefGoogle ScholarPubMed
Trigg, P. I. (1968). A new continuous perfusion technique for the cultivation of malaria parasites in vitro. Transactions of the Royal Society of Tropical Medicine and Hygiene 62, 371–8.CrossRefGoogle Scholar
Trigg, P. I. & Gutteridge, W. E. (1972). A minimal medium for the growth of Plasmodium knowlesi in dilution cultures. Parasitology 62, 113–23.CrossRefGoogle Scholar