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Investigation of the relationship between CE cyst characteristics and genetic diversity of Echinococcus granulosus sensu lato in humans from Turkey

Published online by Cambridge University Press:  17 August 2020

Serra Örsten Open the ORCID record for Serra Örsten [Opens in a new window] ,
Türkmen Çiftçi ,
Aynur Azizova ,
Gökhan Yüce ,
Aycan Uysal ,
Çetin İmamoğlu ,
Ergun Karaağaoğlu ,
Devrim Akıncı ,
Yakut Akyön  and
Adriano Casulli Open the ORCID record for Adriano Casulli [Opens in a new window]
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Serra Örsten*
Affiliation:
Vocational School of Health Services, Hacettepe University, Ankara, Turkey
Türkmen Çiftçi
Affiliation:
Department of Radiology, Faculty of Medicine, Hacettepe University, Ankara, Turkey
Aynur Azizova
Affiliation:
Department of Radiology, Faculty of Medicine, Hacettepe University, Ankara, Turkey
Gökhan Yüce
Affiliation:
Department of Radiology, Ministry of Health, Ankara City Hospital, Ankara, Turkey
Aycan Uysal
Affiliation:
Department of Radiology, Ministry of Health, Gülhane Training and Research Hospital, Ankara, Turkey
Çetin İmamoğlu
Affiliation:
Department of Radiology, Ministry of Health, Dr Abdurrahman Yurtaslan Ankara Oncology Training and Research Hospital, Ankara, Turkey
Ergun Karaağaoğlu
Affiliation:
Department of Biostatistics, Faculty of Medicine, Hacettepe University, Ankara, Turkey
Devrim Akıncı
Affiliation:
Department of Radiology, Faculty of Medicine, Hacettepe University, Ankara, Turkey
Yakut Akyön
Affiliation:
Department of Medical Microbiology, Faculty of Medicine, Hacettepe University, Ankara, Turkey
Adriano Casulli
Affiliation:
WHO Collaborating Centre for the Epidemiology, Detection and Control of Cystic and Alveolar Echinococcosis, Department of Infectious Diseases, Istituto Superiore di Sanità, Rome, Italy European Reference Laboratory for Parasites (EURLP), Department of Infectious Diseases, Istituto Superiore di Sanità, Rome, Italy
Okan Akhan
Affiliation:
Department of Radiology, Faculty of Medicine, Hacettepe University, Ankara, Turkey
*
Author for correspondence: Serra Örsten, E-mail: serra.orsten@hacettepe.edu.tr
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Abstract

Cystic echinococcosis (CE) is one of the most common zoonotic diseases worldwide, particularly in rural areas. This study aimed at the identification of the genotype/species belonging to Echinococcus granulosus sensu lato (s.l.) specimens in retrieved percutaneously from the human host and to investigate their relationship with cyst characteristics. The genetic identification of cyst material was performed by mt-CO1 gene polymerase chain reaction, and confirmed via sequencing. A total of 110 CE cysts were identified as E. granulosus s.l. In detail, 104 belonged to E. granulosus sensu stricto (G1 and G3) and six isolates were in the E. canadensis cluster (G6/7). All clusters were tested for the relationship between demographics, cyst features and genetic diversity. The relationship between genetic variation and certain clinical characteristics such as cyst volume and location were statistically significant for G6/7 cluster. Further studies are required with a larger sample set to investigate the relationship between the genetic variability of E. granulosus s.l. and cyst features.

Keywords

Echinococcus granulosus sensu latogenetic diversityhuman cystic echinococcosisparasitic cyst characteristics
Type
Research Article
Information
Parasitology , Volume 147 , Issue 14 , December 2020 , pp. 1712 - 1717
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Copyright
Copyright © The Author(s), 2020. Published by Cambridge University Press

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