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A kinetoplast DNA probe diagnostic for Leishmania infantum

Published online by Cambridge University Press:  06 April 2009

M. Gramiccia ,
D. F. Smith ,
M. C. Angelici ,
P. D. Ready  and
L. Gradoni
M. Gramiccia
Affiliation:
Laboratorio di Parassitologia, Istituto Superiore di Sanità, vials Regina Elena 299, 00161 Rome, Italy
D. F. Smith
Affiliation:
Department of Biochemistry, Imperial College of Science, Technology and Medicine, London SW7 2AY, U.K.
M. C. Angelici
Affiliation:
Laboratorio di Parassitologia, Istituto Superiore di Sanità, vials Regina Elena 299, 00161 Rome, Italy
P. D. Ready
Affiliation:
Department of Entomology, The Natural History Museum, London SW7 5BD, U.K.
L. Gradoni
Affiliation:
Laboratorio di Parassitologia, Istituto Superiore di Sanità, vials Regina Elena 299, 00161 Rome, Italy
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Extract

A 196 bp fragment of cloned kinetoplast (k) DNA of Leishmania infantum has been sequenced and shown to be diagnostic for this species. The DNA from 104–105 promastigotes was routinely and specifically detected in crude dot blots of whole cells lysed in situ, and there was no cross-hybridization with 106 cells of L. major, L. tropica, L. killicki, L. aethiopica, L. braziliensis and L. mexicana. A low degree of hybridization was found with L. donovani and L. chagasi. This probe will be of particular use in Mediterranean countries, such as Italy, in which dermotropic zymodemes of L. infantum can be difficult to isolate and grow in numbers sufficient for isoenzyme typing.

Keywords

Leishmania infantumkinetoplast DNA probe.
Type
Research Article
Information
Parasitology , Volume 105 , Issue 1 , August 1992 , pp. 29 - 34
Copyright
Copyright © Cambridge University Press 1992

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References

REFERENCES

Angelici, M. C., Gramiccia, M. & Gradoni, L. (1989). Study on genetic polymorphism of Leishmania infantum through the analysis of restriction enzyme digestion patterns of kinetoplast DNA. Parasitology 99, 301–9.CrossRefGoogle Scholar
Barker, D. C., Arnot, D. E. & Butcher, J. (1982). DNA characterisation as a taxonomic tool for identification of kinetoplastic flagellate protozoans. In Biochemical Characterisation of Leishmania (ed. Chance, M. L. & Walton, B. C.), pp. 139–80. Geneva: UNDP/World Bank/WHO.Google Scholar
Barker, D. C., Gibson, L. J., Kennedy, W. P. K., Nasser, A. A. A. A. & Williams, R. H. (1986). The potential of using recombinant DNA species-specific probes for the identification of tropical Leishmania. Parasitology 91, S139–S174.CrossRefGoogle Scholar
Belazzoug, S., Lanotte, G., Maazoun, R., Pratlong, F. & Rioux, J. A. (1985). Un nouveau variant enzymatique de Leishmania infantum Nicolle, 1908, agent de la leishmaniose cutanée du Nord de l'Algérie. Annales de Parasitologie Humaine et Comparée 60, 1–3.CrossRefGoogle Scholar
Bullock, W. O., Fernandez, J. M. & Short, J. M. (1987). XL1-Blue: a high-efficiency plasmid transforming recA Escherichia coli strain with beta-galactosidase selection. Biotechniques 5, 376–8.Google Scholar
Evans, D. A. (1987). Leishmania. In Vitro Methods for Parasite Cultivation (ed. Taylor, A. E. R. & Baker, J. R.), pp. 52–75. London: Academic Press.Google Scholar
Feinberg, A. P. & Vogelstein, B. (1984). A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity. Analytical Biochemistry 137, 266–7.Google ScholarPubMed
Gramiccia, M., Gradoni, L. & Angelici, M. C. (1989). Epidemiology of Mediterranean leishmaniasis caused by Leishmania infantum: isoenzyme and kDNA analysis for the identification of parasites from man, vectors and reservoirs. In Leishmaniasis: the Current Status and New Strategies for Control, NATO-ASI Series, Series A, Vol. 163 (ed. Hart, D. J.), pp. 21–37. New York: Plenum Press.CrossRefGoogle Scholar
Gramiccia, M., Gradoni, L. & Pozio, E. (1987). Leishmania infantum sensu lato as an agent of cutaneous leishmaniasis in Abruzzi region (Italy). Transactions of the Royal Society of Tropical Medicine and Hygiene 81, 235–7.CrossRefGoogle ScholarPubMed
Gramiccia, M., Gradoni, L. & Troiani, M. (1991). HIV-Leishmania co-infections in Italy: isoenzyme characterization of Leishmania causing visceral leishmaniasis in HIV patients. Transactions of the Royal Society of Tropical Medicine and Hygiene 85, (in the Press).Google Scholar
Howard, M. K., Kelly, J. M., Lane, R. P. & Miles, M. A. (1991). A sensitive repetitive DNA probe that is specific to the Leishmania donovani complex and its use as an epidemiological and diagnostic reagent. Molecular and Biochemical Parasitology 44, 63–72.CrossRefGoogle Scholar
Joliffe, D. S., Evans, D. A. & Bryceson, A. D. M. (1986). Cutaneous leishmaniasis. British Journal of Dermatology 114, 745–6.CrossRefGoogle Scholar
Morel, C., Chiari, E., Plessmann Camargo, E., Mattei, D. M., Romanha, A. J. & Simpson, L. (1980). Strains and clones of Trypanosoma cruzi can be characterized by pattern of restriction endonuclease products of kinetoplast DNA minicircles. Proceedings of the National Academy of Sciences, USA 77, 6810–14.CrossRefGoogle ScholarPubMed
Moreno, G., Rioux, J. A., Lanotte, G., Pratlong, F. & Serres, E. (1986). Le complexe Leishmania donovani s.l. Analyse enzymatique et traitement numérique. Individualisation du complexe Leishmania infantum. Corollaires biogéographiques et phylétiques. À propos de 146 souches originaires de l'Ancien et du Nouveau Monde. In Leishmania. Taxonomie et Phylogénèse. Applications Eco-Epedémiologiques. (Coll. Int. CNRS/INSERM, 1984), (ed. Rioux, J. A.), pp. 105–17. Paris: CNRS/INSERM.Google Scholar
Pietrzak Rohrer, S., Michelotti, E. F., Torri, A. F. & Hajduk, S. L. (1987). Transcription of kinetoplast DNA minicircles. Cell 49, 625–32.CrossRefGoogle Scholar
Rioux, J. A., Lanotte, G., Dedet, J. P. & Martini-Dumas, J. A. (1970). Utilisation du milieu ‘coeur-cerveau-sang de mouton’ pour la culture en masse des formes promastigotes des Leishmanies. Annales de Parasitologie Humaine et Comparée 45, 381–4.CrossRefGoogle Scholar
Rioux, J. A., Lanotte, G. & Pratlong, F. (1986). Leishmania killicki n.sp. (Kinetoplastida, Trypanosomatidae). In Leishmania. Taxonomie et Phyiogénèse. Applications Eco-Epidémiologiques. (Coll. Int. CNRS/INSERM, 1984). (Ed. Rioux, J. A.), pp. 139–42. Paris: CNRS/INSERM.Google Scholar
Rioux, J. A., Lanotte, G., Maazoun, R., Perello, R. & Pratlong, F. (1980). Leishmania infantum Nicolle, 1908, agent du Bouton d'Orient autochtone. À propos de l'identification biochimique de deux souches isolées dans les Pyrénées-Orientales. Comptes Rendus des Séances de l'Académie des Sciences, Paris 291, Series D, 701–3.Google Scholar
Rioux, J. A., Lanotte, G., Petter, F., Dereure, J, Akalay, O., Pratlong, F., Velez, I. D., Fikri, N. B., Maazoun, R., Denial, M., Jarry, D. M., Zahaf, A., Ashford, R. W., Cadi-Soussi, M., Killick-Kendrick, R., Benmansour, N., Moreno, G., Perieres, J., Guilvard, E., Zribi, N., Kennou, M. F., Rispail, P., Knechtli, R. & Serres, E. (1986). Les leishmanioses cutanées du bassin Méditerranéen occidental. De l'identification enzymatique à l'analyse éco-épidémiologique. L'exemple de trois ‘foyers’, tunisien, marocain et français. In Leishmania. Taxonomie et Phylogénèse. Applications Eco-Epidémiologiques. (Coll. Int. CNRS/INSERM, 1984). (Ed. Rioux, J. A.), pp. 365–95. Paris: CNRS/INSERM.Google Scholar
Rogers, W. O. & Wirth, D. F. (1987). Kinetoplast DNA minicircles: regions of extensive sequence divergence. Proceedings of the National Academy of Sciences, USA 84, 565–9.CrossRefGoogle ScholarPubMed
Sambrook, J., Fritsch, E. F. & Maniatis, T. (1989). Molecular Cloning. A Laboratory Manual, 2nd Edn. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press.Google Scholar
Sanger, F., Nicklen, S. & Coulson, A. R. (1977). DNA sequencing with chain termination inhibitors. Proceedings of the National Academy of Sciences, USA 74, 5463–7.CrossRefGoogle ScholarPubMed
Smith, D. F., Searle, S., Ready, P. D., Gramiccia, M. & Ben-Ismail, R. (1989). A kinetoplast DNA probe diagnostic for Leishmania major: sequence homologies between regions of Leishmania minicircles. Molecular and Biochemical Parasitology 37, 213–24.CrossRefGoogle ScholarPubMed
Van Eys, G. J. J. M., Schoone, G. J., Ligthart, G. S., Alvar, J., Evans, D. A. & Terpstra, W. J. (1989). Identification of ‘Old World’ Leishmania by DNA recombinant probes. Molecular and Biochemical Parasitology 34, 53–62.CrossRefGoogle ScholarPubMed