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Kinetoplast minicircle DNA from Leishmania (Viannia) lainsoni

Published online by Cambridge University Press:  01 April 1999

S. H. E. McCANN ,
S. ERESH  and
D. C. BARKER
S. H. E. McCANN
Affiliation:
Molteno Laboratories, Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP
S. ERESH
Affiliation:
Molteno Laboratories, Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP
D. C. BARKER
Affiliation:
Molteno Laboratories, Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP
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Abstract

To investigate the phenomenon that PCR of Leishmania (V.) lainsoni minicircles using primers B1 and B2 gives anomalous small-sized products, unlike all other members of the Leishmania Viannia subgenus, cloned kDNA minicircles from L. (Viannia) lainsoni were sequenced using fluorescent dye terminator reactions. The sequence of L. (V.) lainsoni where the primer B2 would be expected to bind, was different from the other members of the L. Viannia subgenus, matching in only 7 out of 19 bases with the sequence of L. (V.) braziliensis at this position. The sequence obtained from the cloned minicircles enabled the design of a new primer which, when combined with B1, allowed the amplification of full sized minicircles in L. (V.) lainsoni, but not other members of the L. Viannia subgenus. Comparison of the sequence obtained for Leishmania (V.) lainsoni with other Leishmania minicircle DNA confirms that Leishmania (V.) lainsoni is more similar to members of the L. Viannia subgenus than to other Leishmania, but is distinctly different.

Keywords

Leishmania (V.) lainsoniminicircle DNAPCR
Type
Research Article
Information
Parasitology , Volume 118 , Issue 4 , April 1999 , pp. 371 - 374
Copyright
© 1999 Cambridge University Press

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Footnotes

Complete minicircle sequences submitted to GENBANK with the accession numbers AFO88225-88235. Small PCR product sequences submitted to GENBANK with the accession numbers AFO93691-93695 AFO96162-4.