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Longitudinal studies of the immune response of Colombian patients infected with Trypanosoma cruzi and T. rangeli

Published online by Cambridge University Press:  06 April 2009

L. Hudson ,
F. Guhl ,
N. de Sanchez ,
D. Bridge ,
C. A. Jaramillo  and
A. Young
L. Hudson
Affiliation:
Department of Immunology, St George's Hospital Medical School, Cranmer Terrace, London SW17 ORE
F. Guhl
Affiliation:
Departmento de Ciencias Biologicas, Universidad de los Andes, Bogota D.E., Colombia
N. de Sanchez
Affiliation:
Departmento de Ciencias Biologicas, Universidad de los Andes, Bogota D.E., Colombia
D. Bridge
Affiliation:
Department of Immunology, St George's Hospital Medical School, Cranmer Terrace, London SW17 ORE
C. A. Jaramillo
Affiliation:
Departmento de Ciencias Biologicas, Universidad de los Andes, Bogota D.E., Colombia
A. Young
Affiliation:
Department of Immunology, St George's Hospital Medical School, Cranmer Terrace, London SW17 ORE
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Summary

Two groups of patients were examined for anti-Trypanosoma cruzi antibodies by immunofluorescence and ELISA (i) inhabitants of the village and surrounding rural area of Tibu, Norte de Santander, Colombia (n = 327) and (ii) employees of the Empresa Colombiana de Petroleos (ECOPETROL, n = 849). The latter group had a lower rate of positive serology (12 as compared to 29%) but the distributions of antibody titres were very similar in the two groups. A total of 119 serum samples (37 village and 82 ECOPETROL, including 25 seronegative controls) were analysed for their ability to immunoprecipitate the 7 major polypeptides of T. cruzi trypomastigotes of Mr > 72 kDa. Although 10 sera from positive patients showed no immunoprecipitation, all of the remaining positive sera contained antibodies which reacted with the 150, 90 and 85 kDa polypeptides. When the T. cruzi immunofluorescence positive, immunoprecipitation negative sera were retested by ELISA using GP90, all were negative thus suggesting that the patients had had a misdiagnosed T. rangeli infection. The new diagnosis was confirmed by immunofluorescence and ELISA with T. rangeli epimastigotes. Longitudinal studies were carried out on 19 patients from the ECOPETROL group for up to 3–5 years. Five seropositive patients showed a change in their anti-trypomastigote immunoprecipitation profiles over this period; one by loss of a previously recognized high molecular weight band and four others by conversion from a negative to a positive immunoprecipitation profile. These latter patients presented initially with uncomplicated T. rangeli infection but then acquired a T. cruzi superinfection. These patients represent the nucleus of a group in which prospective studies will identify the effect of T. rangeli infection on the course of subsequent South American trypanosomiasis and Chagas' disease.

Type
Research Article
Information
Parasitology , Volume 96 , Issue 3 , June 1988 , pp. 449 - 460
Copyright
Copyright © Cambridge University Press 1988

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