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Low temperature preservation of Plasmodium spp

Published online by Cambridge University Press:  06 April 2009

S. M. Mutetwa  and
E. R. James
S. M. Mutetwa
Affiliation:
309 Roma Court, Salisbury Street at Fife Avenue, Harare, Zimbabwe
E. R. James*
Affiliation:
Department of Ophthalmology, Medical University of South Carolina, 171 Ashley Avenue, Charleston, SC 29425, USA
*
*For correspondence.
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Extract

Interest in the long-term low temperature preservation of malaria parasites was initiated by Coggeshall's (1939) demonstration that the intra-erythrocytic stages could survive storage at −76°C for 70 days. Numerous studies have subsequently been published in which a variety of techniques have been used. In addition, several reviews have included sections on the cryopreservation of malaria, the most recent being by James (1980), Leef, Hollingdale, & Beaudoin (1981), Nguyen-Dinh, Chemangey-Masaba, Spencer, Campbell, Chin & Collins (1981) and a WHO memorandum (1981). Most of these reports and reviews have dealt with the problems of cryopreserving malaria parasites from a parasitological viewpoint. In an attempt to provide a cryobiologically orientated review of value both to cryobiologists and practicing parasitologists the published technical reports have been summarized in chronological order in Tables 1 and 2, and the important cryobiological parameters are discussed.

Type
Trends and Perspectives
Information
Parasitology , Volume 90 , Issue 3 , June 1985 , pp. 589 - 603
Copyright
Copyright © Cambridge University Press 1985

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References

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