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Mechanisms of mortality in Culicoides biting midges due to Haemoproteus infection

Published online by Cambridge University Press:  09 September 2016

DOVILĖ BUKAUSKAITĖ ,
RASA BERNOTIENĖ ,
TATJANA A. IEZHOVA  and
GEDIMINAS VALKIŪNAS
DOVILĖ BUKAUSKAITĖ*
Affiliation:
Institute of Ecology, Nature Research Centre, Akademijos 2, LT-08412 Vilnius, Lithuania
RASA BERNOTIENĖ
Affiliation:
Institute of Ecology, Nature Research Centre, Akademijos 2, LT-08412 Vilnius, Lithuania
TATJANA A. IEZHOVA
Affiliation:
Institute of Ecology, Nature Research Centre, Akademijos 2, LT-08412 Vilnius, Lithuania
GEDIMINAS VALKIŪNAS
Affiliation:
Institute of Ecology, Nature Research Centre, Akademijos 2, LT-08412 Vilnius, Lithuania
*
*Corresponding author: Institute of Ecology, Nature Research Centre, Akademijos 2, LT-08412 Vilnius, Lithuania. E-mail: dovilebu7@gmail.com
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Summary

We examined the effects of Haemoproteus infection on the survival and pathology caused in the biting midges. Forty-six females of Culicoides impunctatus were exposed experimentally by allowing them to feed on a naturally infected red-backed shrike infected with Haemoproteus lanii (lineage hRB1, gametocytaemia 5·2%). Seventeen females were fed on an uninfected bird (controls). Dead insects were collected, counted and used for dissection, histological examination and polymerase chain reaction-based testing. Parasites were present in all experimentally infected biting midges, but absent from control insects. Survivorship differed significantly between the control and infected groups. Twelve hours post-exposure (PE), 45 (98%) experimentally infected midges were dead, but all control midges remained alive, and many of them survived until 7 day PE. The migrating ookinetes of H. lanii overfilled midgut, markedly damaged the midgut wall, entered the haemocoel and overfilled the abdomen and thorax of exposed biting midges. Massive infection by migrating ookinetes led to damage of abdomen and thorax of biting midges. The parasites often present in large clumps in the haemocoel in abdomen and thorax, leading to the interruption of the haemolymph circulation. These are the main reasons for rapid death of biting midges after feeding on high-intensity infections of Haemoproteus parasites.

Keywords

Haemosporidian parasitesHaemoproteusCulicoidesbiting midgesmortalityhistologyookinetes
Type
Research Article
Information
Parasitology , Volume 143 , Issue 13 , November 2016 , pp. 1748 - 1754
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Copyright
Copyright © Cambridge University Press 2016 

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