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Molecular and immunological characterization of L14 ribosomal protein from Leishmania braziliensis

Published online by Cambridge University Press:  01 March 2004

A. C. GONZÁLEZ ,
M. C. THOMAS ,
E. MARTÍNEZ-CARRETERO ,
E. CARMELO ,
M. C. LÓPEZ  and
B. VALLADARES
A. C. GONZÁLEZ
Affiliation:
Departamento de Parasitología. Facultad de Farmacia, Universidad de La Laguna. Avda. Francisco Sánchez s/n. C.P. 38271 La Laguna, Tenerife, Spain
M. C. THOMAS
Affiliation:
Unidad Mixta de Investigaciones Médicas. Hospital Universitario San Cecilio, SAS, Avda. Dr. Oloriz 16, C.P. 18012 Granada, Spain
E. MARTÍNEZ-CARRETERO
Affiliation:
Departamento de Parasitología. Facultad de Farmacia, Universidad de La Laguna. Avda. Francisco Sánchez s/n. C.P. 38271 La Laguna, Tenerife, Spain
E. CARMELO
Affiliation:
Departamento de Parasitología. Facultad de Farmacia, Universidad de La Laguna. Avda. Francisco Sánchez s/n. C.P. 38271 La Laguna, Tenerife, Spain
M. C. LÓPEZ
Affiliation:
Departamento de Biología Molecular, Instituto de Parasitología y Biomedicina ‘López Neyra’, CSIC, Calle Ventanilla 11, 18001 Granada, Spain
B. VALLADARES
Affiliation:
Departamento de Parasitología. Facultad de Farmacia, Universidad de La Laguna. Avda. Francisco Sánchez s/n. C.P. 38271 La Laguna, Tenerife, Spain
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Abstract

The isolation and molecular characterization of the gene coding for L14 ribosomal protein from L. braziliensis is described. There are 2 copies of the gene per haploid genome, repeated in a head-to-tail tandem orientation and located in a single chromosome of approximately 950 kb. Northern blot analyses indicate the presence of a single transcript of 0·95 kb which is up-regulated when parasites reach the stationary growth phase. L. braziliensis L14 gene codes for a 175 amino acid long polypeptide showing 75–83% sequence identity with L14 proteins from trypanosomatids and approximately 25% with its counterparts from higher eukaryotic organisms. L14 ribosomal proteins from trypanosomatids and higher eukaryotes share along their molecules a similar distribution pattern of theoretically functional domains. L. braziliensis L14 recombinant protein is not recognized by sera from cutaneous leishmaniasis patients. Immunization of mice with one dose of L14 recombinant protein and a second dose of L14 protein covalently linked to the HSP70 from Trypanosoma cruzi induces a high antibody level against this L14 protein, which is mostly of the IgG2a subtype, as well as a strong increase in splenocyte proliferation index.

Keywords

Leishmania braziliensisL14 ribosomal proteinimmunogenicity
Type
Research Article
Information
Parasitology , Volume 128 , Issue 2 , February 2004 , pp. 139 - 147
Copyright
2004 Cambridge University Press

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